8S55

RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state a (composite structure)

8S55 の概要

• エントリーDOI: 10.2210/pdb8s55/pdb
• EMDBエントリー: 19726
• 分子名称: DNA-directed RNA polymerase subunit, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11-a, ... (21 entities in total)
• 機能のキーワード: rna polymerase ii, promoter escape, de novo transcription, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 797592.78

構造登録者

Zhan, Y.,Grabbe, F.,Oberbeckmann, E.,Dienemann, C.,Cramer, P. (登録日: 2024-02-22, 公開日: 2024-04-17, 最終更新日: 2024-11-06)

主引用文献

Zhan, Y.,Grabbe, F.,Oberbeckmann, E.,Dienemann, C.,Cramer, P.
Three-step mechanism of promoter escape by RNA polymerase II.
Mol.Cell, 84:1699-1710.e6, 2024

PubMed Abstract: The transition from transcription initiation to elongation is highly regulated in human cells but remains incompletely understood at the structural level. In particular, it is unclear how interactions between RNA polymerase II (RNA Pol II) and initiation factors are broken to enable promoter escape. Here, we reconstitute RNA Pol II promoter escape in vitro and determine high-resolution structures of initially transcribing complexes containing 8-, 10-, and 12-nt ordered RNAs and two elongation complexes containing 14-nt RNAs. We suggest that promoter escape occurs in three major steps. First, the growing RNA displaces the B-reader element of the initiation factor TFIIB without evicting TFIIB. Second, the rewinding of the transcription bubble coincides with the eviction of TFIIA, TFIIB, and TBP. Third, the binding of DSIF and NELF facilitates TFIIE and TFIIH dissociation, establishing the paused elongation complex. This three-step model for promoter escape fills a gap in our understanding of the initiation-elongation transition of RNA Pol II transcription.

PubMed: 38604172
DOI: 10.1016/j.molcel.2024.03.016

実験手法

ELECTRON MICROSCOPY (3.4 Å)