8S4S

PrgE from plasmid pCF10

8S4S の概要

• エントリーDOI: 10.2210/pdb8s4s/pdb
• 分子名称: PrgE, SULFATE ION, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: ssb, dna binding protein
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34366.95

構造登録者

Breidenstein, A.,Berntsson, R.P.-A. (登録日: 2024-02-22, 公開日: 2024-05-29, 最終更新日: 2024-07-03)

主引用文献

Breidenstein, A.,Lamy, A.,Bader, C.P.,Sun, W.S.,Wanrooij, P.H.,Berntsson, R.P.
PrgE: an OB-fold protein from plasmid pCF10 with striking differences to prototypical bacterial SSBs.
Life Sci Alliance, 7:-, 2024

PubMed Abstract: A major pathway for horizontal gene transfer is the transmission of DNA from donor to recipient cells via plasmid-encoded type IV secretion systems (T4SSs). Many conjugative plasmids encode for a single-stranded DNA-binding protein (SSB) together with their T4SS. Some of these SSBs have been suggested to aid in establishing the plasmid in the recipient cell, but for many, their function remains unclear. Here, we characterize PrgE, a proposed SSB from the plasmid pCF10. We show that PrgE is not essential for conjugation. Structurally, it has the characteristic OB-fold of SSBs, but it has very unusual DNA-binding properties. Our DNA-bound structure shows that PrgE binds ssDNA like beads on a string supported by its N-terminal tail. In vitro studies highlight the plasticity of PrgE oligomerization and confirm the importance of the N-terminus. Unlike other SSBs, PrgE binds both double- and single-stranded DNA equally well. This shows that PrgE has a quaternary assembly and DNA-binding properties that are very different from the prototypical bacterial SSB, but also different from eukaryotic SSBs.

PubMed: 38811160
DOI: 10.26508/lsa.202402693

実験手法

X-RAY DIFFRACTION (2.7 Å)