8S3E

Structure of rabbit Slo1 in complex with gamma1/LRRC26

8S3E の概要

• エントリーDOI: 10.2210/pdb8s3e/pdb
• EMDBエントリー: 19691
• 分子名称: Calcium-activated potassium channel subunit alpha-1, Leucine-rich repeat-containing protein 26, CHOLESTEROL, ... (8 entities in total)
• 機能のキーワード: ion channel, potassium transport, membrane protein
• 由来する生物種: Oryctolagus cuniculus (rabbit); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 682105.46

構造登録者

Redhardt, M.,Raunser, S.,Raisch, T. (登録日: 2024-02-20, 公開日: 2024-04-10, 最終更新日: 2024-05-01)

主引用文献

Redhardt, M.,Raunser, S.,Raisch, T.
Cryo-EM structure of the Slo1 potassium channel with the auxiliary gamma 1 subunit suggests a mechanism for depolarization-independent activation.
Febs Lett., 598:875-888, 2024

PubMed Abstract: Mammalian Ca-dependent Slo K channels can stably associate with auxiliary γ subunits which fundamentally alter their behavior. By a so far unknown mechanism, the four γ subunits reduce the need for voltage-dependent activation and, thereby, allow Slo to open independently of an action potential. Here, using cryo-EM, we reveal how the transmembrane helix of γ1/LRRC26 binds and presumably stabilizes the activated voltage-sensor domain of Slo1. The activation is further enhanced by an intracellular polybasic stretch which locally changes the charge gradient across the membrane. Our data provide a possible explanation for Slo1 regulation by the four γ subunits and also their different activation efficiencies. This suggests a novel activation mechanism of voltage-gated ion channels by auxiliary subunits.

PubMed: 38553946
DOI: 10.1002/1873-3468.14863

実験手法

ELECTRON MICROSCOPY (2.39 Å)