8S37
DNA-bound Type IV-A3 CRISPR effector in complex with DinG helicase from K. pneumoniae (state III)
Summary for 8S37
| Entry DOI | 10.2210/pdb8s37/pdb |
| EMDB information | 19045 19688 19689 19690 |
| Descriptor | CRISPR type AFERR-associated protein Csf2, CRISPR type AFERR-associated protein Csf3, CRISPR type AFERR-associated protein Csf1, ... (8 entities in total) |
| Functional Keywords | crispr, crrna, dna binding, type iv crispr-cas, crispri, nuclease deficient, gene regulation, antiviral protein |
| Biological source | Klebsiella pneumoniae More |
| Total number of polymer chains | 12 |
| Total formula weight | 413955.41 |
| Authors | Skorupskaite, A.,Ragozius, V.,Cepaite, R.,Klein, N.,Randau, L.,Malinauskaite, L.,Pausch, P. (deposition date: 2024-02-19, release date: 2024-11-06) |
| Primary citation | Cepaite, R.,Klein, N.,Miksys, A.,Camara-Wilpert, S.,Ragozius, V.,Benz, F.,Skorupskaite, A.,Becker, H.,Zvejyte, G.,Steube, N.,Hochberg, G.K.A.,Randau, L.,Pinilla-Redondo, R.,Malinauskaite, L.,Pausch, P. Structural variation of types IV-A1- and IV-A3-mediated CRISPR interference. Nat Commun, 15:9306-9306, 2024 Cited by PubMed Abstract: CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulation and plasmid competition. To understand how the type IV-A system associated effector complex achieves this interference, we determine cryo-EM structures of two evolutionarily distinct type IV-A complexes (types IV-A1 and IV-A3) bound to cognate DNA-targets in the presence and absence of the type IV-A signature DinG effector helicase. The structures reveal how the effector complexes recognize the protospacer adjacent motif and target-strand DNA to form an R-loop structure. Additionally, we reveal differences between types IV-A1 and IV-A3 in DNA interactions and structural motifs that allow for in trans recruitment of DinG. Our study provides a detailed view of type IV-A mediated DNA-interference and presents a structural foundation for engineering type IV-A-based genome editing tools. PubMed: 39468082DOI: 10.1038/s41467-024-53778-1 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.9 Å) |
Structure validation
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