8RXX

CRYO-EM STRUCTURE OF LEISHMANIA MAJOR 80S RIBOSOME WITH A/P/E-site tRNA AND mRNA : LM32Cs3H1 sKO STRAIN

This is a non-PDB format compatible entry.

Summary for 8RXX

• Entry DOI: 10.2210/pdb8rxx/pdb
• EMDB information: 19582
• Descriptor: LSUa_rRNA_chain_1, Putative ribosomal protein L3, Putative ribosomal protein L1a, ... (94 entities in total)
• Functional Keywords: cryo-em, leishmania major, 80s, ribosome
• Biological source: Leishmania major strain Friedlin; More
• Total number of polymer chains: 89
• Total formula weight: 3620226.22

Authors

Rajan, K.S.,Yonath, A. (deposition date: 2024-02-08, release date: 2024-05-15, Last modification date: 2024-10-23)

Primary citation

Rajan, K.S.,Aryal, S.,Hiregange, D.G.,Bashan, A.,Madmoni, H.,Olami, M.,Doniger, T.,Cohen-Chalamish, S.,Pescher, P.,Taoka, M.,Nobe, Y.,Fedorenko, A.,Bose, T.,Zimermann, E.,Prina, E.,Aharon-Hefetz, N.,Pilpel, Y.,Isobe, T.,Unger, R.,Spath, G.F.,Yonath, A.,Michaeli, S.
Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.
Cell Rep, 43:114203-114203, 2024

PubMed Abstract: Leishmania is the causative agent of cutaneous and visceral diseases affecting millions of individuals worldwide. Pseudouridine (Ψ), the most abundant modification on rRNA, changes during the parasite life cycle. Alterations in the level of a specific Ψ in helix 69 (H69) affected ribosome function. To decipher the molecular mechanism of this phenotype, we determine the structure of ribosomes lacking the single Ψ and its parental strain at ∼2.4-3 Å resolution using cryo-EM. Our findings demonstrate the significance of a single Ψ on H69 to its structure and the importance for its interactions with helix 44 and specific tRNAs. Our study suggests that rRNA modification affects translation of mRNAs carrying codon bias due to selective accommodation of tRNAs by the ribosome. Based on the high-resolution structures, we propose a mechanism explaining how the ribosome selects specific tRNAs.

PubMed: 38722744
DOI: 10.1016/j.celrep.2024.114203

Experimental method

ELECTRON MICROSCOPY (2.97 Å)