8RVC

Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to ketoarginine

8RVC の概要

• エントリーDOI: 10.2210/pdb8rvc/pdb
• 関連するPDBエントリー: 8R4Z
• 分子名称: 2-ketoarginine methyltransferase, 5-[(diaminomethylidene)amino]-2-oxopentanoic acid, MAGNESIUM ION, ... (8 entities in total)
• 機能のキーワード: s adenosylmethionine-dependent methyltransferases, biocatalysis, c-alkylation, asymmetric methylation, mutagenesis, transferase
• 由来する生物種: Pseudomonas syringae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83185.55

構造登録者

Gerhardt, S.,Kemper, F.,Andexer, J.N. (登録日: 2024-02-01, 公開日: 2024-07-03, 最終更新日: 2024-10-16)

主引用文献

Sommer-Kamann, C.,Breiltgens, J.,Zou, Z.,Gerhardt, S.,Saleem-Batcha, R.,Kemper, F.,Einsle, O.,Andexer, J.N.,Muller, M.
Structures and Protein Engineering of the alpha-Keto Acid C-Methyltransferases SgvM and MrsA for Rational Substrate Transfer.
Chembiochem, 25:e202400258-e202400258, 2024

PubMed Abstract: S-adenosyl-l-methionine-dependent methyltransferases (MTs) are involved in the C-methylation of a variety of natural products. The MTs SgvM from Streptomyces griseoviridis and MrsA from Pseudomonas syringae pv. syringae catalyze the methylation of the β-carbon atom of α-keto acids in the biosynthesis of the antibiotic natural products viridogrisein and 3-methylarginine, respectively. MrsA shows high substrate selectivity for 5-guanidino-2-oxovalerate, while other α-keto acids, such as the SgvM substrates 4-methyl-2-oxovalerate, 2-oxovalerate, and phenylpyruvate, are not accepted. Here we report the crystal structures of SgvM and MrsA in the apo form and bound with substrate or S-adenosyl-l-methionine. By investigating key residues for substrate recognition in the active sites of both enzymes and engineering MrsA by site-directed mutagenesis, the substrate range of MrsA was extended to accept α-keto acid substrates of SgvM with uncharged and lipophilic β-residues. Our results showcase the transfer of the substrate scope of α-keto acid MTs from different biosynthetic pathways by rational design.

PubMed: 38887142
DOI: 10.1002/cbic.202400258

実験手法

X-RAY DIFFRACTION (1.969 Å)