8RT8

Conformation-C of the full-length outer membrane core complex (TrwH/VirB7, TrwF/VirB9, TrwE/VirB10CTD) from the fully-assembled R388 type IV secretion system determined by cryo-EM.

8RT8 の概要

• エントリーDOI: 10.2210/pdb8rt8/pdb
• EMDBエントリー: 19482
• 分子名称: TrwE protein, TrwF protein, TrwH protein (3 entities in total)
• 機能のキーワード: type iv secretion system type 4 secretion system t4ss omcc conformation-c core complex outer membrane complex r388 plasmid conjugation bacterial secretion secretion secretion system protein complex virb10 virb9 virb7 trwe trwf trwh, membrane protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 46
• 化学式量合計: 1226340.61

構造登録者

Mace, K.,Waksman, G. (登録日: 2024-01-25, 公開日: 2024-06-19, 最終更新日: 2024-10-23)

主引用文献

Mace, K.,Waksman, G.
Cryo-EM structure of a conjugative type IV secretion system suggests a molecular switch regulating pilus biogenesis.
Embo J., 43:3287-3306, 2024

PubMed Abstract: Conjugative type IV secretion systems (T4SS) mediate bacterial conjugation, a process that enables the unidirectional exchange of genetic materials between a donor and a recipient bacterial cell. Bacterial conjugation is the primary means by which antibiotic resistance genes spread among bacterial populations (Barlow 2009; Virolle et al, 2020). Conjugative T4SSs form pili: long extracellular filaments that connect with recipient cells. Previously, we solved the cryo-electron microscopy (cryo-EM) structure of a conjugative T4SS. In this article, based on additional data, we present a more complete T4SS cryo-EM structure than that published earlier. Novel structural features include details of the mismatch symmetry within the OMCC, the presence of a fourth VirB8 subunit in the asymmetric unit of both the arches and the inner membrane complex (IMC), and a hydrophobic VirB5 tip in the distal end of the stalk. Additionally, we provide previously undescribed structural insights into the protein VirB10 and identify a novel regulation mechanism of T4SS-mediated pilus biogenesis by this protein, that we believe is a key checkpoint for this process.

PubMed: 38886579
DOI: 10.1038/s44318-024-00135-z

実験手法

ELECTRON MICROSCOPY (3.05 Å)