8ROT

Cryo-EM structure of the transmembrane anti-sigma factor DdvA

8ROT の概要

• エントリーDOI: 10.2210/pdb8rot/pdb
• EMDBエントリー: 19404
• 分子名称: CHAT domain-containing protein (1 entity in total)
• 機能のキーワード: transmembrane protein, anti-sigma factor, antiviral system, tpr-chat, signaling protein
• 由来する生物種: Myxococcus xanthus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 217281.17

構造登録者

Lopez-Alonso, J.P.,Ochoa-Lizarralde, B.,Tascon, I.,Ubarretxena-Belandia, I. (登録日: 2024-01-12, 公開日: 2024-11-06, 最終更新日: 2024-11-27)

主引用文献

Bernal-Bernal, D.,Pantoja-Uceda, D.,Lopez-Alonso, J.P.,Lopez-Rojo, A.,Lopez-Ruiz, J.A.,Galbis-Martinez, M.,Ochoa-Lizarralde, B.,Tascon, I.,Elias-Arnanz, M.,Ubarretxena-Belandia, I.,Padmanabhan, S.
Structural basis for regulation of a CBASS-CRISPR-Cas defense island by a transmembrane anti-sigma factor and its ECF sigma partner.
Sci Adv, 10:eadp1053-eadp1053, 2024

PubMed Abstract: How CRISPR-Cas and cyclic oligonucleotide-based antiphage signaling systems (CBASS) are coordinately deployed against invaders remains unclear. We show that a locus containing two CBASS and one type III-B CRISPR-Cas system, regulated by the transmembrane anti-σ DdvA and its cognate extracytoplasmic function (ECF) σ DdvS, can defend against a phage. Cryo-electron microscopy reveals DdvA-DdvS pairs assemble as arrow-shaped transmembrane dimers. Each DdvA periplasmic domain adopts a separase/craspase-type tetratricopeptide repeat (TPR)-caspase HetF-associated with TPR (TPR-CHAT) architecture with an incomplete His-Cys active site, lacking three α-helices conserved among CHAT domains. Each active site faces the dimer interface, raising the possibility that signal-induced caspase-like DdvA autoproteolysis in trans precedes RseP-mediated intramembrane proteolysis and DdvS release. Nuclear magnetic resonance reveals a DdvA cytoplasmic CHCC-type zinc-bound three-helix bundle that binds to DdvS σ and σ domains, undergoing σ-induced helix extension to trap DdvS. Altogether, we provide structural-mechanistic insights into membrane anti-σ-ECF σ regulation of an antiviral CBASS-CRISPR-Cas defense island.

PubMed: 39454004
DOI: 10.1126/sciadv.adp1053

実験手法

ELECTRON MICROSCOPY (3.11 Å)