8RNJ

Unspecific peroxygenase from Marasmius wettsteinii (MweUPO-1)

8RNJ の概要

• エントリーDOI: 10.2210/pdb8rnj/pdb
• 分子名称: Unspecific peroxygenase, MAGNESIUM ION, SULFATE ION, ... (8 entities in total)
• 機能のキーワード: oxidoreductase, peroxygenase, peroxidase
• 由来する生物種: Marasmius wettsteinii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54958.55

構造登録者

Fernandez-Garcia, A.,Sanz-Aparicio, J. (登録日: 2024-01-10, 公開日: 2024-10-16, 最終更新日: 2024-10-30)

主引用文献

Sanchez-Moreno, I.,Fernandez-Garcia, A.,Mateljak, I.,Gomez de Santos, P.,Hofrichter, M.,Kellner, H.,Sanz-Aparicio, J.,Alcalde, M.
Structural Insights and Reaction Profile of a New Unspecific Peroxygenase from Marasmius wettsteinii Produced in a Tandem-Yeast Expression System.
Acs Chem.Biol., 19:2240-2253, 2024

PubMed Abstract: Fungal unspecific peroxygenases (UPOs) are gaining momentum in synthetic chemistry. Of special interest is the UPO from (UPO), which shows an exclusive repertoire of oxyfunctionalizations, including the terminal hydroxylation of alkanes, the α-oxidation of fatty acids and the C-C cleavage of corticosteroids. However, the lack of heterologous expression systems to perform directed evolution has impeded its engineering for practical applications. Here, we introduce a close ortholog of UPO, a UPO gene from (UPO-1), that has a similar reaction profile to UPO and for which we have set up a directed evolution platform based on tandem-yeast expression. Recombinant UPO-1 was produced at high titers in the bioreactor (0.7 g/L) and characterized at the biochemical and atomic levels. The conjunction of soaking crystallographic experiments at a resolution up to 1.6 Å together with the analysis of reaction patterns sheds light on the substrate preferences of this promiscuous biocatalyst.

PubMed: 39367827
DOI: 10.1021/acschembio.4c00504

実験手法

X-RAY DIFFRACTION (1.6 Å)