8RM7

Crystal Structure of Human Androgen Receptor DNA Binding Domain Bound to its Response Element: MMTV-177 GRE/ARE

8RM7 の概要

• エントリーDOI: 10.2210/pdb8rm7/pdb
• 分子名称: Isoform 2 of Androgen receptor, MMTV-177 GRE/ARE Chain C, MMTV-177 GRE/ARE, Chain D, ... (5 entities in total)
• 機能のキーワード: dbd-dna binding complex, androgen receptor dna binding domain, structural protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 27379.67

構造登録者

Lee, X.Y.,Helsen, C.,Van Eynde, W.,Voet, A.,Claessens, F. (登録日: 2024-01-05, 公開日: 2024-04-24)

主引用文献

Lee, X.Y.,Van Eynde, W.,Helsen, C.,Willems, H.,Peperstraete, K.,De Block, S.,Voet, A.,Claessens, F.
Structural mechanism underlying variations in DNA binding by the androgen receptor.
J.Steroid Biochem.Mol.Biol., 241:106499-106499, 2024

PubMed Abstract: The androgen receptor (AR) is a steroid activated transcription factor which recognizes DNA motifs resembling inverted repeats of a conserved 5'-AGAACA-3'-like hexanucleotides separated by a three-nucleotide spacer from a similar, but less conserved hexanucleotide. Here, we report the structures of the human AR DNA binding domain (DBD) bound to two natural AREs (C3 and MTV) in head-to-head dimer conformations, diffracting at 2.05 Å and 2.25 Å, respectively. These structures help to explain the impact of androgen insensitivity mutations on the structure integrity, DNA binding and DBD dimerization. The binding affinity of the AR DBD to different DNA motifs were measured by the BioLayer Interferometry (BLI) and further validated by Molecular Dynamics (MD) simulations. This shows that the high binding affinity of the first DBD to the upstream 5'-AGAACA-3' motif induces the cooperative binding of the second DBD to the second hexanucleotide. Our data indicate identical interaction of the DBDs to the upstream hexanucleotides, while forming an induced closer contact of the second DBD on the non-canonical hexanucleotides. The variation in binding between the DBD monomers are the result of differences in DNA occupancy, protein-protein interactions, DNA binding affinity, and DNA binding energy profiles. We propose this has functional consequences.

PubMed: 38604378
DOI: 10.1016/j.jsbmb.2024.106499

実験手法

X-RAY DIFFRACTION (2.25 Å)