8RJW

Human RAD52 open ring - ssDNA complex

8RJW の概要

• エントリーDOI: 10.2210/pdb8rjw/pdb
• 関連するPDBエントリー: 8RIL 8RJ3
• EMDBエントリー: 19253
• 分子名称: DNA repair protein RAD52 homolog, ssDNA, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: ssdna binding protein, dna damage repair, single-strand annealing, dna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 423166.91

構造登録者

Liang, C.C.,West, S.C. (登録日: 2023-12-21, 公開日: 2024-04-24, 最終更新日: 2024-05-29)

主引用文献

Liang, C.C.,Greenhough, L.A.,Masino, L.,Maslen, S.,Bajrami, I.,Tuppi, M.,Skehel, M.,Taylor, I.A.,West, S.C.
Mechanism of single-stranded DNA annealing by RAD52-RPA complex.
Nature, 629:697-703, 2024

PubMed Abstract: RAD52 is important for the repair of DNA double-stranded breaks, mitotic DNA synthesis and alternative telomere length maintenance. Central to these functions, RAD52 promotes the annealing of complementary single-stranded DNA (ssDNA) and provides an alternative to BRCA2/RAD51-dependent homologous recombination repair. Inactivation of RAD52 in homologous-recombination-deficient BRCA1- or BRCA2-defective cells is synthetically lethal, and aberrant expression of RAD52 is associated with poor cancer prognosis. As a consequence, RAD52 is an attractive therapeutic target against homologous-recombination-deficient breast, ovarian and prostate cancers. Here we describe the structure of RAD52 and define the mechanism of annealing. As reported previously, RAD52 forms undecameric (11-subunit) ring structures, but these rings do not represent the active form of the enzyme. Instead, cryo-electron microscopy and biochemical analyses revealed that ssDNA annealing is driven by RAD52 open rings in association with replication protein-A (RPA). Atomic models of the RAD52-ssDNA complex show that ssDNA sits in a positively charged channel around the ring. Annealing is driven by the RAD52 N-terminal domains, whereas the C-terminal regions modulate the open-ring conformation and RPA interaction. RPA associates with RAD52 at the site of ring opening with critical interactions occurring between the RPA-interacting domain of RAD52 and the winged helix domain of RPA2. Our studies provide structural snapshots throughout the annealing process and define the molecular mechanism of ssDNA annealing by the RAD52-RPA complex.

PubMed: 38658755
DOI: 10.1038/s41586-024-07347-7

実験手法

ELECTRON MICROSCOPY (2.3 Å)