8RJT

Serial femtosecond X-ray structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in I6 intermediate state.

8RJT の概要

• エントリーDOI: 10.2210/pdb8rjt/pdb
• 関連するPDBエントリー: 6G1Y 6G1Z 6G20 8RJM 8RJN 8RJO 8RJP 8RJQ 8RJR 8RJS 8RJU
• 分子名称: histidine kinase, 3-[(2Z)-2-({3-(2-carboxyethyl)-5-[(E)-(4-ethenyl-3-methyl-5-oxo-1,5-dihydro-2H-pyrrol-2-ylidene)methyl]-4-methyl-1H-pyrrol-2-yl}methylidene)-5-{(Z)-[(3E,4S)-3-ethylidene-4-methyl-5-oxopyrrolidin-2-ylidene]methyl}-4-methyl-2H-pyrrol-3-yl]propanoic acid, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: fluorescent protein, phytochrome, bacteriophytochrome, intermediate, optogenetic, signaling protein
• 由来する生物種: Agrobacterium fabrum str. C58
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 116988.25

構造登録者

Sauthof, L.,Schmidt, A.,Szczepek, M.,Brewster, A.S.,Kern, J.F.,Scheerer, P. (登録日: 2023-12-21, 公開日: 2025-05-14)

• 主引用文献: Sauthof, L.,Szczepek, M.,Schmidt, A.,Bhowmick, A.,Dasgupta, M.,Mackintosh, M.J.,Gul, S.,Fuller, F.D.,Chatterjee, R.,Young, I.D.,Michael, N.,Heyder, N.A.,Bauer, B.,Koch, A.,Bogacz, I.,Kim, I.-S.,Simon, P.S.,Butryn, A.,Aller, P.,Chukhutsina, V.U.,Baxter, J.M.,Hutchison, C.D.M.,Liebschner, D.,Poon, B.,Sauter, N.K.,Miller, M.D.,Phillips Jr., G.N.,Alonso-Mori, R.,Hunter, M.S.,Batyuk, A.,Owada, S.,Tono, K.,Tanaka, R.,van Thor, J.J.,Krauss, N.,Lamparter, T.,Brewster, A.S.,Schapiro, I.,Orville, A.M.,Yachandra, V.K.,Yano, J.,Hildebrandt, P.,Kern, J.F.,Scheerer, P.; Serial-femtosecond crystallography reveals how a phytochrome variant couples chromophore and protein structural changes; Sci Adv, 2025

実験手法

X-RAY DIFFRACTION (2.49 Å)