8RJD

Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 2

これはPDB形式変換不可エントリーです。

8RJD の概要

• エントリーDOI: 10.2210/pdb8rjd/pdb
• EMDBエントリー: 19198
• 分子名称: Protein transport protein Sec61 subunit alpha isoform 1, Ribosomal protein L8, Nascent chain, ... (58 entities in total)
• 機能のキーワード: ribosome, membrane protein, translocon, transport
• 由来する生物種: Bos taurus; 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 2366694.06

構造登録者

Lewis, A.J.O.,Hegde, R.S. (登録日: 2023-12-20, 公開日: 2024-01-24, 最終更新日: 2024-11-13)

主引用文献

Lewis, A.J.O.,Zhong, F.,Keenan, R.J.,Hegde, R.S.
Structural analysis of the dynamic ribosome-translocon complex.
Elife, 13:-, 2024

PubMed Abstract: The protein translocon at the endoplasmic reticulum comprises the Sec61 translocation channel and numerous accessory factors that collectively facilitate the biogenesis of secretory and membrane proteins. Here, we leveraged recent advances in cryo-electron microscopy (cryo-EM) and structure prediction to derive insights into several novel configurations of the ribosome-translocon complex. We show how a transmembrane domain (TMD) in a looped configuration passes through the Sec61 lateral gate during membrane insertion; how a nascent chain can bind and constrain the conformation of ribosomal protein uL22; and how the translocon-associated protein (TRAP) complex can adjust its position during different stages of protein biogenesis. Most unexpectedly, we find that a large proportion of translocon complexes contains RAMP4 intercalated into Sec61's lateral gate, widening Sec61's central pore and contributing to its hydrophilic interior. These structures lead to mechanistic hypotheses for translocon function and highlight a remarkably plastic machinery whose conformations and composition adjust dynamically to its diverse range of substrates.

PubMed: 38896445
DOI: 10.7554/eLife.95814

実験手法

ELECTRON MICROSCOPY (2.78574 Å)