8RI6

Beta-galactosidase (LacZ) purified from E. coli.

8RI6 の概要

• エントリーDOI: 10.2210/pdb8ri6/pdb
• EMDBエントリー: 19181
• 分子名称: Beta-galactosidase, MAGNESIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: beta-galactosidase, inhibitor, iminosugar, plant pathogenic bacteria., hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 469967.61

構造登録者

Hardenbrook, N.J.,Sheng, Y.,Zhang, P. (登録日: 2023-12-18, 公開日: 2025-01-01, 最終更新日: 2026-04-01)

主引用文献

Sanguankiattichai, N.,Chandrasekar, B.,Sheng, Y.,Hardenbrook, N.,Tabak, W.W.A.,Drapal, M.,Kaschani, F.,Grunwald-Gruber, C.,Krahn, D.,Buscaill, P.,Yamamoto, S.,Kato, A.,Nash, R.,Fleet, G.,Strasser, R.,Fraser, P.D.,Kaiser, M.,Zhang, P.,Preston, G.M.,van der Hoorn, R.A.L.
Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology.
Science, 388:297-303, 2025

PubMed Abstract: The extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen pv. DC3000 produces glycosyrin. Glycosyrin inhibits the plant-secreted β-galactosidase BGAL1, which would otherwise initiate the release of immunogenic peptides from bacterial flagellin. Here, we report the structure, biosynthesis, and multifunctional roles of glycosyrin. High-resolution cryo-electron microscopy and chemical synthesis revealed that glycosyrin is an iminosugar with a five-membered pyrrolidine ring and a hydrated aldehyde that mimics monosaccharides. Glycosyrin biosynthesis was controlled by virulence regulators, and its production is common in bacteria and prevents flagellin recognition and alters the extracellular glycoproteome and metabolome of infected plants. These findings highlight a potentially wider role for glycobiology manipulation by plant pathogens across the plant kingdom.

PubMed: 40245141
DOI: 10.1126/science.adp2433

実験手法

ELECTRON MICROSCOPY (2.06 Å)