8RFE

CgsiGP2 sample in nanodisc

8RFE の概要

• エントリーDOI: 10.2210/pdb8rfe/pdb
• EMDBエントリー: 19118
• 分子名称: Cyclic beta 1-2 glucan synthetase, beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose, URIDINE-5'-DIPHOSPHATE-GLUCOSE (3 entities in total)
• 機能のキーワード: cgs, cyclic glucan, antibiotic
• 由来する生物種: Agrobacterium tumefaciens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 316534.84

構造登録者

Sedzicki, J.,Ni, D.,Lehmann, F.,Stahlberg, H.,Dehio, C. (登録日: 2023-12-12, 公開日: 2024-03-20, 最終更新日: 2025-07-09)

主引用文献

Sedzicki, J.,Ni, D.,Lehmann, F.,Stahlberg, H.,Dehio, C.
Structure-function analysis of the cyclic beta-1,2-glucan synthase from Agrobacterium tumefaciens.
Nat Commun, 15:1844-1844, 2024

PubMed Abstract: The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans (Brucella) and plants (Agrobacterium). CβG is produced by the cyclic glucan synthase (Cgs), a multi-domain membrane protein. So far, its structure as well as the mechanism underlining the synthesis have not been clarified. Here we use cryo-electron microscopy (cryo-EM) and functional approaches to study Cgs from A. tumefaciens. We determine the structure of this complex protein machinery and clarify key aspects of CβG synthesis, revealing a distinct mechanism that uses a tyrosine-linked oligosaccharide intermediate in cycles of polymerization and processing of the glucan chain. Our research opens possibilities for combating pathogens that rely on polysaccharide virulence factors and may lead to synthetic biology approaches for producing complex cyclic sugars.

PubMed: 38418509
DOI: 10.1038/s41467-024-45415-8

実験手法

ELECTRON MICROSCOPY (3.8 Å)