8RBS

Emiliania huxleyi virus 201 (EhV-201) asymmetrical unit of capsid proteins predicted by AlphaFold2 fitted into the cryo-EM density of EhV-201 virion composite map.

これはPDB形式変換不可エントリーです。

「8PFN」から置き換えられました

8RBS の概要

• エントリーDOI: 10.2210/pdb8rbs/pdb
• EMDBエントリー: 19035
• 分子名称: Major capsid protein, Penton protein (2 entities in total)
• 機能のキーワード: cryo-em, subtomogram averaging, ehv-201, enveloped virus, capsid, major capsid protein, composite map, virus
• 由来する生物種: Emiliania huxleyi virus 201; 詳細
• タンパク質・核酸の鎖数: 85
• 化学式量合計: 4705085.95

構造登録者

Homola, M.,Buttner, C.R.,Fuzik, T.,Novacek, J.,Chaillet, M.,Forster, F.,Plevka, P. (登録日: 2023-12-04, 公開日: 2023-12-20, 最終更新日: 2024-04-24)

主引用文献

Homola, M.,Buttner, C.R.,Fuzik, T.,Krepelka, P.,Holbova, R.,Novacek, J.,Chaillet, M.L.,Zak, J.,Grybchuk, D.,Forster, F.,Wilson, W.H.,Schroeder, D.C.,Plevka, P.
Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi.
Sci Adv, 10:eadk1954-eadk1954, 2024

PubMed Abstract: The globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect by using fivefold vertices to bind to and fuse the virus' inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum-derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from cells, which enables the continuous release of virions by exocytosis.

PubMed: 38598627
DOI: 10.1126/sciadv.adk1954

実験手法

ELECTRON MICROSCOPY (18 Å)