8RAY

ParA in complex with ATP

8RAY の概要

• エントリーDOI: 10.2210/pdb8ray/pdb
• 分子名称: ParA family protein, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: soj, dna replication, replication
• 由来する生物種: Myxococcus xanthus DK 1622
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57135.56

構造登録者

Mais, C.-N.,Bange, G. (登録日: 2023-12-01, 公開日: 2025-03-26, 最終更新日: 2025-10-08)

主引用文献

Schnabel, L.,Osorio-Valeriano, M.,Perez-Borrajero, C.,Steinchen, W.,Mais, C.N.,Simon, B.,Hanssmann, J.,Thamm, M.,Hennig, J.,Bange, G.,Thanbichler, M.
Molecular basis of ParA ATPase activation by the CTPase ParB during bacterial chromosome segregation.
Nat Commun, 16:8428-8428, 2025

PubMed Abstract: DNA segregation by bacterial ParABS systems is mediated by transient tethering interactions between nucleoid-bound dimers of the ATPase ParA and centromere (parS)-associated complexes of the clamp-forming CTPase ParB. The lifetime of these interactions is limited by the ParB-dependent activation of ParA ATPase activity. Here, we elucidate the functional interplay between ParA and ParB in the model bacterium Myxococcus xanthus. We demonstrate that the N-terminal ParA-binding motif of ParB associates with a conserved bipartite binding pocket at the ParA dimer interface, in a manner dependent on ParB clamp closure. Moreover, we show that ParB and non-specific DNA interact cooperatively with ParA and synergistically induce structural changes in its Walker A and Walker B motifs that correlate with the activation of ParA ATPase activity. These results advance our understanding of the mechanism underlying DNA transport by the ParABS system and may help to unravel the mode of action of related cargo-positioning systems.

PubMed: 40998818
DOI: 10.1038/s41467-025-63976-0

実験手法

X-RAY DIFFRACTION (1.58 Å)