8RAP

Structure of Sen1-ADP.BeF3 bound RNA Polymerase II pre-termination complex

8RAP の概要

• エントリーDOI: 10.2210/pdb8rap/pdb
• EMDBエントリー: 19022
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (23 entities in total)
• 機能のキーワード: rna polymerase ii, pol ii, termination, sen1, gene regulation
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 958356.50

構造登録者

Rengachari, S.,Lidscreiber, M.,Cramer, P. (登録日: 2023-12-01, 公開日: 2024-10-30, 最終更新日: 2025-02-26)

主引用文献

Rengachari, S.,Hainthaler, T.,Oberthuer, C.,Lidschreiber, M.,Cramer, P.
Mechanism of polyadenylation-independent RNA polymerase II termination.
Nat.Struct.Mol.Biol., 32:339-345, 2025

PubMed Abstract: The mechanisms underlying the initiation and elongation of RNA polymerase II (Pol II) transcription are well-studied, whereas termination remains poorly understood. Here we analyze the mechanism of polyadenylation-independent Pol II termination mediated by the yeast Sen1 helicase. Cryo-electron microscopy structures of two pretermination intermediates show that Sen1 binds to Pol II and uses its adenosine triphosphatase activity to pull on exiting RNA in the 5' direction. This is predicted to push Pol II forward, induce an unstable hypertranslocated state and destabilize the transcription bubble, thereby facilitating termination. This mechanism of transcription termination may be widely used because it is conceptually conserved in the bacterial transcription system.

PubMed: 39424994
DOI: 10.1038/s41594-024-01409-0

実験手法

ELECTRON MICROSCOPY (4.3 Å)