8R7Y

Deoxyribonucleoside regulator DeoR in complex with the DNA operator

8R7Y の概要

• エントリーDOI: 10.2210/pdb8r7y/pdb
• 分子名称: Deoxyribonucleoside regulator, OL18 DNA operator, strand 1, OL18 DNA operator, strand 2, ... (4 entities in total)
• 機能のキーワード: transcriptional repressor, protein-dna complex, bacillus subtilis, dna binding protein
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 165239.19

構造登録者

Pachl, P.,Soltysova, M.,Rezacova, P. (登録日: 2023-11-27, 公開日: 2024-06-19, 最終更新日: 2024-07-17)

主引用文献

Soltysova, M.,Skerlova, J.,Pachl, P.,Skubnik, K.,Fabry, M.,Sieglova, I.,Farolfi, M.,Grishkovskaya, I.,Babiak, M.,Novacek, J.,Krasny, L.,Rezacova, P.
Structural characterization of two prototypical repressors of SorC family reveals tetrameric assemblies on DNA and mechanism of function.
Nucleic Acids Res., 52:7305-7320, 2024

PubMed Abstract: The SorC family of transcriptional regulators plays a crucial role in controlling the carbohydrate metabolism and quorum sensing. We employed an integrative approach combining X-ray crystallography and cryo-electron microscopy to investigate architecture and functional mechanism of two prototypical representatives of two sub-classes of the SorC family: DeoR and CggR from Bacillus subtilis. Despite possessing distinct DNA-binding domains, both proteins form similar tetrameric assemblies when bound to their respective DNA operators. Structural analysis elucidates the process by which the CggR-regulated gapA operon is derepressed through the action of two effectors: fructose-1,6-bisphosphate and newly confirmed dihydroxyacetone phosphate. Our findings provide the first comprehensive understanding of the DNA binding mechanism of the SorC-family proteins, shedding new light on their functional characteristics.

PubMed: 38842936
DOI: 10.1093/nar/gkae434

実験手法

X-RAY DIFFRACTION (3.7 Å)