8R4C

LRR domain of Roco protein from C. tepidum bound to the activating Nanobody NbRoco2

Summary for 8R4C

• Entry DOI: 10.2210/pdb8r4c/pdb
• Related: 8R4B
• EMDB information: 18879 18884 18885
• Descriptor: NbRoco2, Rab family protein (2 entities in total)
• Functional Keywords: gtpase, nanobody, parkinson's disease, allostery activator, hydrolase
• Biological source: Lama glama; More
• Total number of polymer chains: 2
• Total formula weight: 138494.20

Authors

Galicia, C.,Versees, W. (deposition date: 2023-11-13, release date: 2024-05-01, Last modification date: 2024-05-15)

Primary citation

Galicia, C.,Guaitoli, G.,Fislage, M.,Gloeckner, C.J.,Versees, W.
Structural insights into the GTP-driven monomerization and activation of a bacterial LRRK2 homolog using allosteric nanobodies.
Elife, 13:-, 2024

PubMed Abstract: Roco proteins entered the limelight after mutations in human LRRK2 were identified as a major cause of familial Parkinson's disease. LRRK2 is a large and complex protein combining a GTPase and protein kinase activity, and disease mutations increase the kinase activity, while presumably decreasing the GTPase activity. Although a cross-communication between both catalytic activities has been suggested, the underlying mechanisms and the regulatory role of the GTPase domain remain unknown. Several structures of LRRK2 have been reported, but structures of Roco proteins in their activated GTP-bound state are lacking. Here, we use single-particle cryo-electron microscopy to solve the structure of a bacterial Roco protein (CtRoco) in its GTP-bound state, aided by two conformation-specific nanobodies: Nb and Nb. This structure presents CtRoco in an active monomeric state, featuring a very large GTP-induced conformational change using the LRR-Roc linker as a hinge. Furthermore, this structure shows how Nb and Nb collaborate to activate CtRoco in an allosteric way. Altogether, our data provide important new insights into the activation mechanism of Roco proteins, with relevance to LRRK2 regulation, and suggest new routes for the allosteric modulation of their GTPase activity.

PubMed: 38666771
DOI: 10.7554/eLife.94503

Experimental method

ELECTRON MICROSCOPY (3.55 Å)