8R3C

Cocrystal form II of the Pent - sulfonato-calix[8]arene complex

8R3C の概要

• エントリーDOI: 10.2210/pdb8r3c/pdb
• 関連するPDBエントリー: 8R3B 8R3D
• 分子名称: Beta propeller, 2-acetamido-2-deoxy-alpha-D-glucopyranose, sulfonato-calix[8]arene, ... (4 entities in total)
• 機能のキーワード: complex, beta propeller, mutivalent, synthetic construct, sugar binding protein
• 由来する生物種: Enterobacteria phage L1
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 56981.36

構造登録者

Flood, R.J.,Crowley, P.B. (登録日: 2023-11-08, 公開日: 2024-03-06)

主引用文献

Flood, R.J.,Cerofolini, L.,Fragai, M.,Crowley, P.B.
Multivalent Calixarene Complexation of a Designed Pentameric Lectin.
Biomacromolecules, 25:1303-1309, 2024

PubMed Abstract: We describe complex formation between a designed pentameric β-propeller and the anionic macrocycle sulfonato-calix[8]arene (), as characterized by X-ray crystallography and NMR spectroscopy. Two crystal structures and N HSQC experiments reveal a single calixarene binding site in the concave pocket of the β-propeller toroid. Despite the symmetry mismatch between the pentameric protein and the octameric macrocycle, they form a high affinity multivalent complex, with the largest protein-calixarene interface observed to date. This system provides a platform for investigating multivalency.

PubMed: 38227741
DOI: 10.1021/acs.biomac.3c01280

実験手法

X-RAY DIFFRACTION (1.58 Å)