8QZK

Catalytic core of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) concieved by deep network hallucination: dEngBF4 Hexagonal form

これはPDB形式変換不可エントリーです。

8QZK の概要

• エントリーDOI: 10.2210/pdb8qzk/pdb
• 関連するPDBエントリー: 8QYE
• 分子名称: ENDO-ALPHA-N-ACETYLGALACTOSAMINIDASE (2 entities in total)
• 機能のキーワード: catalytic core; endo-alpha-n-acetylgalactosaminidase; deep network hallucination; dengbf4, hydrolase; (beta/alpha)-8 barrel, hydrolase
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32822.18

構造登録者

Aghajari, N.,Hansen, A.L.,Thiesen, F.F.,Crehuet, R.,Marcos, E.,Willemoes, M. (登録日: 2023-10-27, 公開日: 2024-03-06, 最終更新日: 2024-10-16)

主引用文献

Hansen, A.L.,Theisen, F.F.,Crehuet, R.,Marcos, E.,Aghajari, N.,Willemoes, M.
Carving out a Glycoside Hydrolase Active Site for Incorporation into a New Protein Scaffold Using Deep Network Hallucination.
Acs Synth Biol, 13:862-875, 2024

PubMed Abstract: Enzymes are indispensable biocatalysts for numerous industrial applications, yet stability, selectivity, and restricted substrate recognition present limitations for their use. Despite the importance of enzyme engineering in overcoming these limitations, success is often challenged by the intricate architecture of enzymes derived from natural sources. Recent advances in computational methods have enabled the de novo design of simplified scaffolds with specific functional sites. Such scaffolds may be advantageous as platforms for enzyme engineering. Here, we present a strategy for the de novo design of a simplified scaffold of an endo-α--acetylgalactosaminidase active site, a glycoside hydrolase from the GH101 enzyme family. Using a combination of trRosetta hallucination, iterative cycles of deep-learning-based structure prediction, and ProteinMPNN sequence design, we designed proteins with 290 amino acids incorporating the active site while reducing the molecular weight by over 100 kDa compared to the initial endo-α--acetylgalactosaminidase. Of 11 tested designs, six were expressed as soluble monomers, displaying similar or increased thermostabilities compared to the natural enzyme. Despite lacking detectable enzymatic activity, the experimentally determined crystal structures of a representative design closely matched the design with a root-mean-square deviation of 1.0 Å, with most catalytically important side chains within 2.0 Å. The results highlight the potential of scaffold hallucination in designing proteins that may serve as a foundation for subsequent enzyme engineering.

PubMed: 38357862
DOI: 10.1021/acssynbio.3c00674

実験手法

X-RAY DIFFRACTION (3.25 Å)