8QWQ
Magic-angle spinning NMR Structure of Opa60 in Lipid Bilayers
Summary for 8QWQ
| Entry DOI | 10.2210/pdb8qwq/pdb |
| NMR Information | BMRB: 34872 |
| Descriptor | Opacity protein opA60 (Fragment) (1 entity in total) |
| Functional Keywords | structure from cyana 3.98.15, beta barrel, bacterial outer membrane, cell adhesion |
| Biological source | Neisseria gonorrhoeae |
| Total number of polymer chains | 1 |
| Total formula weight | 28772.99 |
| Authors | |
| Primary citation | Forster, M.C.,Tekwani Movellan, K.,Najbauer, E.E.,Becker, S.,Andreas, L.B. Magic-angle spinning NMR structure of Opa60 in lipid bilayers. J Struct Biol X, 9:100098-100098, 2024 Cited by PubMed Abstract: Here we report the structure of Opa60 in lipid bilayers using proton-detected magic-angle spinning nuclear magnetic resonance (MAS NMR). Preparations including near-native oligosaccharide lipids reveal a consistent picture of a stable transmembrane beta barrel with a minor increase in the structured region as compared with the previously reported detergent structure. The large variable loops known to interact with host proteins could not be detected, confirming their dynamic nature even in a lipid bilayer environment. The structure provides a starting point for investigation of the functional role of Opa60 in gonococcal infection, which is understood to involve interaction with host proteins. At the same time, it demonstrates the recent advances in proton-detected methodology for membrane protein structure determination at atomic resolution by MAS NMR. PubMed: 39010882DOI: 10.1016/j.yjsbx.2024.100098 PDB entries with the same primary citation |
| Experimental method | SOLID-STATE NMR |
Structure validation
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