8QWQ

Magic-angle spinning NMR Structure of Opa60 in Lipid Bilayers

8QWQ の概要

• エントリーDOI: 10.2210/pdb8qwq/pdb
• NMR情報: BMRB: 34872
• 分子名称: Opacity protein opA60 (Fragment) (1 entity in total)
• 機能のキーワード: structure from cyana 3.98.15, beta barrel, bacterial outer membrane, cell adhesion
• 由来する生物種: Neisseria gonorrhoeae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28772.99

構造登録者

Forster, M.C.,Andreas, L.B. (登録日: 2023-10-19, 公開日: 2024-07-24)

主引用文献

Forster, M.C.,Tekwani Movellan, K.,Najbauer, E.E.,Becker, S.,Andreas, L.B.
Magic-angle spinning NMR structure of Opa60 in lipid bilayers.
J Struct Biol X, 9:100098-100098, 2024

PubMed Abstract: Here we report the structure of Opa60 in lipid bilayers using proton-detected magic-angle spinning nuclear magnetic resonance (MAS NMR). Preparations including near-native oligosaccharide lipids reveal a consistent picture of a stable transmembrane beta barrel with a minor increase in the structured region as compared with the previously reported detergent structure. The large variable loops known to interact with host proteins could not be detected, confirming their dynamic nature even in a lipid bilayer environment. The structure provides a starting point for investigation of the functional role of Opa60 in gonococcal infection, which is understood to involve interaction with host proteins. At the same time, it demonstrates the recent advances in proton-detected methodology for membrane protein structure determination at atomic resolution by MAS NMR.

PubMed: 39010882
DOI: 10.1016/j.yjsbx.2024.100098

実験手法

SOLID-STATE NMR