8QVM

Comparison of room-temperature and cryogenic structures of soluble Epoxide Hydrolase with ligands bound.

8QVM の概要

• エントリーDOI: 10.2210/pdb8qvm/pdb
• 分子名称: Bifunctional epoxide hydrolase 2, TRIETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: inhibitor, serial crystallography, drug discovery, fixed target, room temperature, microcrystals, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 62152.88

構造登録者

Dunge, A.,Uwangue, O.,Phan, C.,Bjelcic, M.,Gunnarsson, J.,Wehlander, G.,Kack, H.,Branden, G. (登録日: 2023-10-18, 公開日: 2024-08-14, 最終更新日: 2024-09-11)

主引用文献

Dunge, A.,Phan, C.,Uwangue, O.,Bjelcic, M.,Gunnarsson, J.,Wehlander, G.,Kack, H.,Branden, G.
Exploring serial crystallography for drug discovery.
Iucrj, 11:831-842, 2024

PubMed Abstract: Structure-based drug design is highly dependent on the availability of structures of the protein of interest in complex with lead compounds. Ideally, this information can be used to guide the chemical optimization of a compound into a pharmaceutical drug candidate. A limitation of the main structural method used today - conventional X-ray crystallography - is that it only provides structural information about the protein complex in its frozen state. Serial crystallography is a relatively new approach that offers the possibility to study protein structures at room temperature (RT). Here, we explore the use of serial crystallography to determine the structures of the pharmaceutical target, soluble epoxide hydrolase. We introduce a new method to screen for optimal microcrystallization conditions suitable for use in serial crystallography and present a number of RT ligand-bound structures of our target protein. From a comparison between the RT structural data and previously published cryo-temperature structures, we describe an example of a temperature-dependent difference in the ligand-binding mode and observe that flexible loops are better resolved at RT. Finally, we discuss the current limitations and potential future advances of serial crystallography for use within pharmaceutical drug discovery.

PubMed: 39072424
DOI: 10.1107/S2052252524006134

実験手法

X-RAY DIFFRACTION (2 Å)