8QRJ

LCC-ICCG PETase mutant H218Y

8QRJ の概要

• エントリーDOI: 10.2210/pdb8qrj/pdb
• 分子名称: Leaf-branch compost cutinase, 1,2-ETHANEDIOL, 3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL, ... (5 entities in total)
• 機能のキーワード: lcc, petase, hydrolase, direct enzyme evolution
• 由来する生物種: unidentified prokaryotic organism
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31840.94

構造登録者

Orr, G.,Niv, Y.,Barakat, M.,Boginya, A.,Dessau, M.,Afriat-Jurnou, L. (登録日: 2023-10-09, 公開日: 2024-09-18, 最終更新日: 2024-11-20)

主引用文献

Orr, G.,Niv, Y.,Barakat, M.,Boginya, A.,Dessau, M.,Afriat-Jurnou, L.
Streamlined screening of extracellularly expressed PETase libraries for improved polyethylene terephthalate degradation.
Biotechnol J, 19:e2400021-e2400021, 2024

PubMed Abstract: Enzyme-mediated polyethylene terephthalate (PET) depolymerization has recently emerged as a sustainable solution for PET recycling. Towards an industrial-scale implementation of this technology, various strategies are being explored to enhance PET depolymerization (PETase) activity and improve enzyme stability, expression, and purification processes. Recently, rational engineering of a known PET hydrolase (LCC-leaf compost cutinase) has resulted in the isolation of a variant harboring four-point mutations (LCC-ICCG), presenting increased PETase activity and thermal stability. Here, we revealed the enzyme's natural extracellular expression and used it to efficiently screen error-prone genetic libraries based on LCC-ICCG for enhanced activity toward consumer-grade PET. Following multiple rounds of mutagenesis and screening, we successfully isolated variants that exhibited up to a 60% increase in PETase activity. Among other mutations, the improved variants showed a histidine to tyrosine substitution at position 218, a residue known to be involved in substrate binding and stabilization. Introducing H218Y mutation on the background of LCC-ICCG (named here LCC-ICCG/H218Y) resulted in a similar level of activity improvement. Analysis of the solved structure of LCC-ICCG/H218Y compared to other known PETases featuring different amino acids at the equivalent position suggests that H218Y substitution promotes enhanced PETase activity. The expression and screening processes developed in this study can be further used to optimize additional enzymatic parameters crucial for efficient enzymatic degradation of consumer-grade PET.

PubMed: 38987219
DOI: 10.1002/biot.202400021

実験手法

X-RAY DIFFRACTION (1.42 Å)