8QPD

Structure of thioredoxin m from pea

8QPD の概要

• エントリーDOI: 10.2210/pdb8qpd/pdb
• NMR情報: BMRB: 52150
• 分子名称: Thioredoxin M-type, chloroplastic (1 entity in total)
• 機能のキーワード: structure from cyana 2.1, redox process, plant protein
• 由来する生物種: Pisum sativum (garden pea)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11992.76

構造登録者

Neira, J.L.,Camara Artigas, A. (登録日: 2023-10-01, 公開日: 2024-02-14, 最終更新日: 2024-03-27)

主引用文献

Neira, J.L.,Palomino-Schatzlein, M.,Rejas, V.,Traverso, J.A.,Rico, M.,Lopez-Gorge, J.,Chueca, A.,Camara-Artigas, A.
Three-dimensional solution structure, dynamics and binding of thioredoxin m from Pisum sativum.
Int.J.Biol.Macromol., 262:129781-129781, 2024

PubMed Abstract: Thioredoxins (TRXs) are ubiquitous small, globular proteins involved in cell redox processes. In this work, we report the solution structure of TRX m from Pisum sativum (pea), which has been determined on the basis of 1444 nuclear Overhauser effect- (NOE-) derived distance constraints. The average pairwise root-mean-square deviation (RMSD) for the 20 best structures for the backbone residues (Val7-Glu102) was 1.42 ± 0.15 Å, and 1.97 ± 0.15 Å when all heavy atoms were considered. The structure corresponds to the typical fold of TRXs, with a central five-stranded β-sheet flanked by four α-helices. Some residues had an important exchange dynamic contribution: those around the active site; at the C terminus of β-strand 3; and in the loop preceding α-helix 4. Smaller NOE values were observed at the N and C-terminal residues forming the elements of the secondary structure or, alternatively, in the residues belonging to the loops between those elements. A peptide derived from pea fructose-1,6-biphosphatase (FBPase), comprising the preceding region to the regulatory sequence of FBPase (residues Glu152 to Gln179), was bound to TRX m with an affinity in the low micromolar range, as measured by fluorescence and NMR titration experiments. Upon peptide addition, the intensities of the cross-peaks of all the residues of TRX m were affected, as shown by NMR. The value of the dissociation constant of the peptide from TRX m was larger than that of the intact FBPase, indicating that there are additional factors in other regions of the polypeptide chain of the latter protein affecting the binding to thioredoxin.

PubMed: 38296131
DOI: 10.1016/j.ijbiomac.2024.129781

実験手法

SOLUTION NMR