8QOC

Crystal structure of Staphylococcus aureus PLP Synthase (Pdx1)

8QOC の概要

• エントリーDOI: 10.2210/pdb8qoc/pdb
• 分子名称: Pyridoxal 5'-phosphate synthase subunit PdxS, PHOSPHATE ION, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: staphylococcus aureus, vitamin b6, plp synthase, oligomeric state, transferase
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 237750.54

構造登録者

Ullah, N.,Wrenger, C.,Betzel, C. (登録日: 2023-09-28, 公開日: 2024-10-02)

主引用文献

Barra, A.L.C.,Ullah, N.,Brognaro, H.,Gutierrez, R.F.,Wrenger, C.,Betzel, C.,Nascimento, A.S.
Structure and dynamics of the staphylococcal pyridoxal 5-phosphate synthase complex reveal transient interactions at the enzyme interface.
J.Biol.Chem., 300:107404-107404, 2024

PubMed Abstract: Infectious diseases are a significant cause of death, and recent studies estimate that common bacterial infectious diseases were responsible for 13.6% of all global deaths in 2019. Among the most significant bacterial pathogens is Staphylococcus aureus, accounting for more than 1.1 million deaths worldwide in 2019. Vitamin biosynthesis has been proposed as a promising target for antibacterial therapy. Here, we investigated the biochemical, structural, and dynamic properties of the enzyme complex responsible for vitamin B6 (pyridoxal 5-phosphate, PLP) biosynthesis in S. aureus, which comprises enzymes SaPdx1 and SaPdx2. The crystal structure of the 24-mer complex of SaPdx1-SaPdx2 enzymes indicated that the S. aureus PLP synthase complex forms a highly dynamic assembly with transient interaction between the enzymes. Solution scattering data indicated that SaPdx2 typically binds to SaPdx1 at a substoichiometric ratio. We propose a structure-based view of the PLP synthesis mechanism initiated with the assembly of SaPLP synthase complex that proceeds in a highly dynamic interaction between Pdx1 and Pdx2. This interface interaction can be further explored as a potentially druggable site for the design of new antibiotics.

PubMed: 38782204
DOI: 10.1016/j.jbc.2024.107404

実験手法

X-RAY DIFFRACTION (2.83 Å)