8QKU

SWR1-nucleosome complex in configuration 1

これはPDB形式変換不可エントリーです。

8QKU の概要

• エントリーDOI: 10.2210/pdb8qku/pdb
• EMDBエントリー: 18471
• 分子名称: Histone H3, RuvB-like protein 1, RuvB-like protein 2, ... (16 entities in total)
• 機能のキーワード: chromatin remodelling complex, nucleosome, protein-dna complex, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 814963.98

構造登録者

Jalal, A.S.B.,Wigley, D.B. (登録日: 2023-09-18, 公開日: 2024-10-02, 最終更新日: 2025-07-02)

主引用文献

Girvan, P.,Jalal, A.S.B.,McCormack, E.A.,Skehan, M.T.,Knight, C.L.,Wigley, D.B.,Rueda, D.S.
Nucleosome flipping drives kinetic proofreading and processivity by SWR1.
Nature, 636:251-257, 2024

PubMed Abstract: The yeast SWR1 complex catalyses the exchange of histone H2A-H2B dimers in nucleosomes, with Htz1-H2B dimers. Here we used single-molecule analysis to demonstrate two-step double exchange of the two H2A-H2B dimers in a canonical yeast nucleosome with Htz1-H2B dimers, and showed that double exchange can be processive without release of the nucleosome from the SWR1 complex. Further analysis showed that bound nucleosomes flip between two states, with each presenting a different face, and hence histone dimer, to SWR1. The bound dwell time is longer when an H2A-H2B dimer is presented for exchange than when presented with an Htz1-H2B dimer. A hexasome intermediate in the reaction is bound to the SWR1 complex in a single orientation with the 'empty' site presented for dimer insertion. Cryo-electron microscopy analysis revealed different populations of complexes showing nucleosomes caught 'flipping' between different conformations without release, each placing a different dimer into position for exchange, with the Swc2 subunit having a key role in this process. Together, the data reveal a processive mechanism for double dimer exchange that explains how SWR1 can 'proofread' the dimer identities within nucleosomes.

PubMed: 39506114
DOI: 10.1038/s41586-024-08152-y

実験手法

ELECTRON MICROSCOPY (3.8 Å)