8QJD

T6SS-linked Rhs repeat protein - Salmonella bongori Rhs-core domain

8QJD の概要

• エントリーDOI: 10.2210/pdb8qjd/pdb
• 分子名称: Salmonella bongori Rhs core domain (residues 360-1420), ZINC ION (3 entities in total)
• 機能のキーワード: rhs, t6ss, paar-rhs, toxin cannister, rhs-repeat, cell invasion
• 由来する生物種: Salmonella bongori N268-08
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 245531.63

構造登録者

Kielkopf, C.S.,Shneider, M.M.,Leiman, P.G.,Taylor, N.M.I. (登録日: 2023-09-13, 公開日: 2024-09-25, 最終更新日: 2024-11-13)

主引用文献

Kielkopf, C.S.,Shneider, M.M.,Leiman, P.G.,Taylor, N.M.I.
T6SS-associated Rhs toxin-encapsulating shells: Structural and bioinformatical insights into bacterial weaponry and self-protection.
Structure, 2024

PubMed Abstract: Bacteria use the type VI secretion system (T6SS) to secrete toxins into pro- and eukaryotic cells via machinery consisting of a contractile sheath and a rigid tube. Rearrangement hotspot (Rhs) proteins represent one of the most common T6SS effectors. The Rhs C-terminal toxin domain displays great functional diversity, while the Rhs core is characterized by YD repeats. We elucidate the Rhs core structures of PAAR- and VgrG-linked Rhs proteins from Salmonella bongori and Advenella mimigardefordensis, respectively. The Rhs core forms a large shell of β-sheets with a negatively charged interior and encloses a large volume. The S. bongori Rhs toxin does not lead to ordered density in the Rhs shell, suggesting the toxin is unfolded. Together with bioinformatics analysis showing that Rhs toxins predominantly act intracellularly, this suggests that the Rhs core functions two-fold, as a safety feature for the producer cell and as delivery mechanism for the toxin.

PubMed: 39481373
DOI: 10.1016/j.str.2024.10.008

実験手法

X-RAY DIFFRACTION (2.203 Å)