8QHH

NMR solution structure of the green kiwi fruit allergen Act d 8.0101

8QHH の概要

• エントリーDOI: 10.2210/pdb8qhh/pdb
• NMR情報: BMRB: 50811,50812
• 分子名称: Bet v 1 related allergen (1 entity in total)
• 機能のキーワード: pr-10 allergen, allergen
• 由来する生物種: Actinidia deliciosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16812.11

構造登録者

Zeindl, R.,Tollinger, M. (登録日: 2023-09-08, 公開日: 2023-11-08)

主引用文献

Zeindl, R.,Tollinger, M.
NMR resonance assignments of the PR-10 allergens Act c 8 and Act d 8 from golden and green kiwifruit.
Biomol NMR Assign, 15:367-371, 2021

PubMed Abstract: Kiwifruits have become one of the most common food sources triggering allergic reactions. In patients suffering from birch pollen related food allergy, reactions result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1, followed by immunological cross-reactivity to structurally homologous proteins in kiwifruit. Clinical symptoms range from scratching and itching of the oral cavity to more severe immunological reactions such as rhino conjunctivitis. In this work we assigned backbone and side chain H, C and N chemical shifts of the 17 kDa PR-10 allergens Act c 8.0101 and Act d 8.0101 from golden (Actinidia chinesis) and green (Actinidia deliciosa) kiwifruit by solution NMR spectroscopy. The chemical shift data confirm the characteristic Bet v 1 fold for both proteins, consisting of a seven-stranded antiparallel β-sheet interrupted by two short α-helices, along with a long C-terminal α-helix. Our data provide the basis for determining the three-dimensional solution structures of these proteins and characterizing their immunological cross-reactivity on a structural basis.

PubMed: 34106433
DOI: 10.1002/prot.20449

実験手法

SOLUTION NMR