8QEV

Crystal structure of ornithine transcarbamylase from Arabidopsis thaliana (AtOTC) in complex with carbamoyl phosphate

8QEV の概要

• エントリーDOI: 10.2210/pdb8qev/pdb
• 分子名称: Ornithine transcarbamylase, chloroplastic, PHOSPHORIC ACID MONO(FORMAMIDE)ESTER, 1,2-ETHANEDIOL, ... (8 entities in total)
• 機能のキーワード: transcarbamylase, carbamoyl transferase, urea cycle, arginine biosynthesis, transferase
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 110190.90

構造登録者

Nielipinski, M.,Pietrzyk-Brzezinska, A.,Sekula, B. (登録日: 2023-09-01, 公開日: 2023-12-06, 最終更新日: 2024-01-17)

主引用文献

Nielipinski, M.,Pietrzyk-Brzezinska, A.J.,Wlodawer, A.,Sekula, B.
Structural analysis and molecular substrate recognition properties of Arabidopsis thaliana ornithine transcarbamylase, the molecular target of phaseolotoxin produced by Pseudomonas syringae .
Front Plant Sci, 14:1297956-1297956, 2023

PubMed Abstract: Halo blight is a plant disease that leads to a significant decrease in the yield of common bean crops and kiwi fruits. The infection is caused by pathovars that produce phaseolotoxin, an antimetabolite which targets arginine metabolism, particularly by inhibition of ornithine transcarbamylase (OTC). OTC is responsible for production of citrulline from ornithine and carbamoyl phosphate. Here we present the first crystal structures of the plant OTC from (OTC). Structural analysis of OTC complexed with ornithine and carbamoyl phosphate reveals that OTC undergoes a significant structural transition when ornithine enters the active site, from the opened to the closed state. In this study we discuss the mode of OTC inhibition by phaseolotoxin, which seems to be able to act only on the fully opened active site. Once the toxin is proteolytically cleaved, it mimics the reaction transition state analogue to fit inside the fully closed active site of OTC. Additionally, we indicate the differences around the gate loop region which rationally explain the resistance of some bacterial OTCs to phaseolotoxin.

PubMed: 38179474
DOI: 10.3389/fpls.2023.1297956

実験手法

X-RAY DIFFRACTION (1.55 Å)