8QDX

E. coli DNA gyrase bound to a DNA crossover

8QDX の概要

• エントリーDOI: 10.2210/pdb8qdx/pdb
• EMDBエントリー: 18342
• 分子名称: DNA gyrase subunit A, DNA gyrase subunit B, DNA minicircle, ... (4 entities in total)
• 機能のキーワード: dna-protein complex, type iia topoisomerase dna gyrase, isomerase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 447056.21

構造登録者

Vayssieres, M.,Lamour, V. (登録日: 2023-08-30, 公開日: 2024-04-10, 最終更新日: 2024-04-24)

主引用文献

Vayssieres, M.,Marechal, N.,Yun, L.,Lopez Duran, B.,Murugasamy, N.K.,Fogg, J.M.,Zechiedrich, L.,Nadal, M.,Lamour, V.
Structural basis of DNA crossover capture by Escherichia coli DNA gyrase.
Science, 384:227-232, 2024

PubMed Abstract: DNA supercoiling must be precisely regulated by topoisomerases to prevent DNA entanglement. The interaction of type IIA DNA topoisomerases with two DNA molecules, enabling the transport of one duplex through the transient double-stranded break of the other, remains elusive owing to structures derived solely from single linear duplex DNAs lacking topological constraints. Using cryo-electron microscopy, we solved the structure of DNA gyrase bound to a negatively supercoiled minicircle DNA. We show how DNA gyrase captures a DNA crossover, revealing both conserved molecular grooves that accommodate the DNA helices. Together with molecular tweezer experiments, the structure shows that the DNA crossover is of positive chirality, reconciling the binding step of gyrase-mediated DNA relaxation and supercoiling in a single structure.

PubMed: 38603484
DOI: 10.1126/science.adl5899

実験手法

ELECTRON MICROSCOPY (3 Å)