8QCE

Dispersin from Lactiplantibacillus paraplantarum DispLp

8QCE の概要

• エントリーDOI: 10.2210/pdb8qce/pdb
• 分子名称: N-acetyl-beta-hexosaminidase, 6-ACETAMIDO-6-DEOXY-CASTANOSPERMINE, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: biofilm degradation, gh20, poly-n-acetylglucosamine, enzyme catalysis, hydrolase
• 由来する生物種: Lactiplantibacillus paraplantarum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75567.05

構造登録者

Males, A.,Moroz, O.V.,Blagova, E.,Munch, A.,Hansen, G.H.,Johansen, A.H.,Ostergaard, L.H.,Segura, D.R.,Eddenden, A.,Due, A.V.,Gudmand, M.,Salomon, J.,Sorensen, S.R.,Cairo, J.L.F.,Pache, R.A.,Vejborg, R.M.,Davies, G.J.,Wilson, K.S. (登録日: 2023-08-25, 公開日: 2025-03-12, 最終更新日: 2025-03-19)

主引用文献

Males, A.,Moroz, O.V.,Blagova, E.,Munch, A.,Hansen, G.H.,Johansen, A.H.,Ostergaard, L.H.,Segura, D.R.,Eddenden, A.,Due, A.V.,Gudmand, M.,Salomon, J.,Sorensen, S.R.,Franco Cairo, J.P.L.,Nitz, M.,Pache, R.A.,Vejborg, R.M.,Bhosale, S.,Vocadlo, D.J.,Davies, G.J.,Wilson, K.S.
Expansion of the diversity of dispersin scaffolds.
Acta Crystallogr D Struct Biol, 81:130-146, 2025

PubMed Abstract: Microorganisms are known to secrete copious amounts of extracellular polymeric substances (EPS) that form complex matrices around the cells to shield them against external stresses, to maintain structural integrity and to influence their environment. Many microorganisms also secrete enzymes that are capable of remodelling or degrading EPS in response to various environmental cues. One key enzyme class is the poly-β-1,6-linked N-acetyl-D-glucosamine (PNAG)-degrading glycoside hydrolases, of which the canonical member is dispersin B (DspB) from CAZy family GH20. We sought to test the hypothesis that PNAG-degrading enzymes would be present across family GH20, resulting in expansion of the sequence and structural space and thus the availability of PNAGases. Phylogenetic analysis revealed that several microorganisms contain potential DspB-like enzymes. Six of these were expressed and characterized, and four crystal structures were determined (two of which were in complex with the established GH20 inhibitor 6-acetamido-6-deoxy-castanospermine and one with a bespoke disaccharide β-1,6-linked thiazoline inhibitor). One enzyme expressed rather poorly, which restricted crystal screening and did not allow activity measurements. Using synthetic PNAG oligomers and MALDI-TOF analysis, two of the five enzymes tested showed preferential endo hydrolytic activity. Their sequences, having only 26% identity to the pioneer enzyme DspB, highlight the considerable array of previously unconsidered dispersins in nature, greatly expanding the range of potential dispersin backbones available for societal application and engineering.

PubMed: 40019001
DOI: 10.1107/S205979832500110X

実験手法

X-RAY DIFFRACTION (1.05 Å)