8QC8

Crystal structure of NAD-dependent glycoside hydrolase from Flavobacterium sp. (strain K172) in complex with co-factor NAD+

8QC8 の概要

• エントリーDOI: 10.2210/pdb8qc8/pdb
• 分子名称: Gfo/Idh/MocA family oxidoreductase, PHOSPHATE ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: oxidoreductive, sulfoquinovose, glycoside hydrolase, hydrolase
• 由来する生物種: Paenarthrobacter ureafaciens
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 174748.92

構造登録者

Pickles, I.B.,Sharma, M.,Davies, G.J. (登録日: 2023-08-25, 公開日: 2023-12-27, 最終更新日: 2024-01-10)

主引用文献

Kaur, A.,Pickles, I.B.,Sharma, M.,Madeido Soler, N.,Scott, N.E.,Pidot, S.J.,Goddard-Borger, E.D.,Davies, G.J.,Williams, S.J.
Widespread Family of NAD + -Dependent Sulfoquinovosidases at the Gateway to Sulfoquinovose Catabolism.
J.Am.Chem.Soc., 145:28216-28223, 2023

PubMed Abstract: The sulfosugar sulfoquinovose (SQ) is produced by photosynthetic plants, algae, and cyanobacteria on a scale of 10 billion tons per annum. Its degradation, which is essential to allow cycling of its constituent carbon and sulfur, involves specialized glycosidases termed sulfoquinovosidases (SQases), which release SQ from sulfolipid glycoconjugates, so SQ can enter catabolism pathways. However, many SQ catabolic gene clusters lack a gene encoding a classical SQase. Here, we report the discovery of a new family of SQases that use an atypical oxidoreductive mechanism involving NAD as a catalytic cofactor. Three-dimensional X-ray structures of complexes with SQ and NAD provide insight into the catalytic mechanism, which involves transient oxidation at C3. Bioinformatic survey reveals this new family of NAD-dependent SQases occurs within sulfoglycolytic and sulfolytic gene clusters that lack classical SQases and is distributed widely including within clade bacteria, suggesting an important contribution to marine sulfur cycling.

PubMed: 38100472
DOI: 10.1021/jacs.3c11126

実験手法

X-RAY DIFFRACTION (2.35 Å)