8QBZ

Crystal structure of the outer membrane decaheme cytochrome MtrC (E344Boc-Lys)

8QBZ の概要

• エントリーDOI: 10.2210/pdb8qbz/pdb
• 関連するPDBエントリー: 4LM8
• 分子名称: Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC, HEME C, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: boc-lys, cytochrome, electron transport., electron transport
• 由来する生物種: Shewanella oneidensis MR-1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 79419.66

構造登録者

Edwards, M.J.,Lockwood, C.J.,Whiting, K.,Nash, B.W.,Butt, J.N.,Clarke, T.A. (登録日: 2023-08-25, 公開日: 2024-09-04, 最終更新日: 2024-10-02)

主引用文献

Lockwood, C.W.J.,Nash, B.W.,Newton-Payne, S.E.,van Wonderen, J.H.,Whiting, K.P.S.,Connolly, A.,Sutton-Cook, A.L.,Crook, A.,Aithal, A.R.,Edwards, M.J.,Clarke, T.A.,Sachdeva, A.,Butt, J.N.
Genetic Code Expansion in Shewanella oneidensis MR-1 Allows Site-Specific Incorporation of Bioorthogonal Functional Groups into a c -Type Cytochrome.
Acs Synth Biol, 13:2833-2843, 2024

PubMed Abstract: Genetic code expansion has enabled cellular synthesis of proteins containing unique chemical functional groups to allow the understanding and modulation of biological systems and engineer new biotechnology. Here, we report the development of efficient methods for site-specific incorporation of structurally diverse noncanonical amino acids (ncAAs) into proteins expressed in the electroactive bacterium MR-1. We demonstrate that the biosynthetic machinery for ncAA incorporation is compatible and orthogonal to the endogenous pathways of MR-1 for protein synthesis, maturation of -type cytochromes, and protein secretion. This allowed the efficient synthesis of a -type cytochrome, MtrC, containing site-specifically incorporated ncAA in MR-1 cells. We demonstrate that site-specific replacement of surface residues in MtrC with ncAAs does not influence its three-dimensional structure and redox properties. We also demonstrate that site-specifically incorporated bioorthogonal functional groups could be used for efficient site-selective labeling of MtrC with fluorophores. These synthetic biology developments pave the way to expand the chemical repertoire of designer proteins expressed in MR-1.

PubMed: 39158169
DOI: 10.1021/acssynbio.4c00248

実験手法

X-RAY DIFFRACTION (1.9 Å)