8Q8H

Crystal Structure of Apo beta-D-GalNAcase from Niabella aurantiaca (Structure 2)

8Q8H の概要

• エントリーDOI: 10.2210/pdb8q8h/pdb
• 関連するPDBエントリー: 8P4L
• 分子名称: beta-D-GalNAcase from Niabella aurantiaca DSM 17617, SULFATE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: beta-galnacase, gh123, niabella, tim-barrel, hydrolase
• 由来する生物種: Niabella aurantiaca DSM 17617
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 252914.97

構造登録者

Morth, J.P.,Moreno Prieto, E.S.,Siebenhaar, S. (登録日: 2023-08-18, 公開日: 2024-05-15)

主引用文献

Moreno Prieto, E.S.,Fjermedal, S.,Siebenhaar, S.,Vuillemin, M.,Holck, J.,Vincentelli, R.,Gippert, G.P.,Wilkens, C.,Morth, J.P.,Henrissat, B.
Characterization and structural study of a novel beta-N-acetylgalactosaminidase from Niabella aurantiaca.
Febs J., 291:1439-1456, 2024

PubMed Abstract: We report here the identification, characterization and three-dimensional (3D) structure determination of NaNga, a newly identified β-N-acetylgalactosaminidase from the Gram-negative soil bacterium Niabella aurantiaca DSM 17617. When recombinantly expressed in Escherichia coli, the enzyme selectively cleaved 4-nitrophenyl-N-acetyl-β-d-galactosamine (pNP-β-d-GalpNAc). The X-ray crystal structure of the protein was refined to 2.5 Å and consists of an N-terminal β-sandwich domain and a (β/α) barrel catalytic domain. Despite a mere 22% sequence identity, the 3D structure of NaNga is similar to those previously determined for family GH123 members, suggesting it also employs the same substrate-assisted catalytic mechanism. Inhibition by N-acetyl-galactosamine thiazoline (GalNAc-thiazoline) supports the suggested mechanism. A phylogenetic analysis of its proximal sequence space shows significant clustering of unknown sequences around NaNga with sufficient divergence with previously identified GH123 members to subdivide this family into distinct subfamilies. Although the actual biological substrate of our enzyme remains unknown, examination of the active site pocket suggests that it may be a β-N-acetylgalactosaminide substituted by a monosaccharide at O-3. Analysis of the genomic context suggests, in turn, that this substituted β-N-acetylgalactosaminide may be appended to a d-arabinan from an environmental Actinomycete.

PubMed: 38129294
DOI: 10.1111/febs.17042

実験手法

X-RAY DIFFRACTION (2.5 Å)