8Q4H

a membrane-bound menaquinol:organohalide oxidoreductase complex RDH complex

8Q4H の概要

• エントリーDOI: 10.2210/pdb8q4h/pdb
• EMDBエントリー: 18148
• 分子名称: Tetrachloroethene reductive dehalogenase, Probable tetrachloroethene reductive dehalogenase membrane anchor protein, (~{Z})-1,2-bis(chloranyl)ethene, ... (7 entities in total)
• 機能のキーワード: rdh menaquinol:organohalide oxidoreductase, electron transport
• 由来する生物種: Desulfitobacterium hafniense TCE1; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 139082.84

構造登録者

Dongchun, N.,Ekundayo, B.,Henning, S.,Julien, M.,Holliger, C.,Cimmino, L. (登録日: 2023-08-07, 公開日: 2023-10-18, 最終更新日: 2023-11-15)

主引用文献

Cimmino, L.,Duarte, A.G.,Ni, D.,Ekundayo, B.E.,Pereira, I.A.C.,Stahlberg, H.,Holliger, C.,Maillard, J.
Structure of a membrane-bound menaquinol:organohalide oxidoreductase.
Nat Commun, 14:7038-7038, 2023

PubMed Abstract: Organohalide-respiring bacteria are key organisms for the bioremediation of soils and aquifers contaminated with halogenated organic compounds. The major players in this process are respiratory reductive dehalogenases, corrinoid enzymes that use organohalides as substrates and contribute to energy conservation. Here, we present the structure of a menaquinol:organohalide oxidoreductase obtained by cryo-EM. The membrane-bound protein was isolated from Desulfitobacterium hafniense strain TCE1 as a PceAB complex catalysing the dechlorination of tetrachloroethene. Two catalytic PceA subunits are anchored to the membrane by two small integral membrane PceB subunits. The structure reveals two menaquinone molecules bound at the interface of the two different subunits, which are the starting point of a chain of redox cofactors for electron transfer to the active site. In this work, the structure elucidates how energy is conserved during organohalide respiration in menaquinone-dependent organohalide-respiring bacteria.

PubMed: 37923808
DOI: 10.1038/s41467-023-42927-7

実験手法

ELECTRON MICROSCOPY (2.83 Å)