8Q3W

ATP-bound IstB in complex to duplex DNA

Summary for 8Q3W

• Entry DOI: 10.2210/pdb8q3w/pdb
• EMDB information: 18136 3031 3032
• Descriptor: Insertion sequence IS5376 putative ATP-binding protein, DNA (48-MER) Target DNA FW, DNA (48-MER) Traget DNA Rv, ... (5 entities in total)
• Functional Keywords: dna transposition, transposon, transpososome, aaa+ atpases, target dna, is21, istb, insertion sequence, dna binding protein, cryo-electron microscopy.
• Biological source: Geobacillus stearothermophilus; More
• Total number of polymer chains: 12
• Total formula weight: 338324.27

Authors

de la Gandara, A.,Spinola-Amilibia, M.,Araujo-Bazan, L.,Nunez-Ramirez, R.,Berger, J.M.,Arias-Palomo, E. (deposition date: 2023-08-04, release date: 2024-07-10)

• Primary citation: de la Gandara, A.,Spinola-Amilibia, M.,Araujo-Bazan, L.,Nunez-Ramirez, R.,Berger, J.M.,Arias-Palomo, E.; Molecular basis for transposase activation by a dedicated AAA+ ATPase.; Nature, 630:1003-1011, 2024; PubMed: 38926614; DOI: 10.1038/s41586-024-07550-6

Experimental method

ELECTRON MICROSCOPY (3.18 Å)