8Q05

Chlorella sorokiniana Rubisco with CsLinker (alpha3-alpha4) bound: D4 symmetry expanded

8Q05 の概要

• エントリーDOI: 10.2210/pdb8q05/pdb
• EMDBエントリー: 18050
• 分子名称: CsLinker (alpha3-alpha4), Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small subunit, chloroplastic, ... (4 entities in total)
• 機能のキーワード: rubisco, plant protein
• 由来する生物種: Chlorella sorokiniana; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 599158.26

構造登録者

Barrett, J.,Blaza, J.N.,Mackinder, L.C.M. (登録日: 2023-07-27, 公開日: 2024-08-07, 最終更新日: 2024-11-27)

主引用文献

Barrett, J.,Naduthodi, M.I.S.,Mao, Y.,Degut, C.,Musial, S.,Salter, A.,Leake, M.C.,Plevin, M.J.,McCormick, A.J.,Blaza, J.N.,Mackinder, L.C.M.
A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage.
Nat.Plants, 10:1801-1813, 2024

PubMed Abstract: CO fixation is commonly limited by inefficiency of the CO-fixing enzyme Rubisco. Eukaryotic algae concentrate and fix CO in phase-separated condensates called pyrenoids, which complete up to one-third of global CO fixation. Condensation of Rubisco in pyrenoids is dependent on interaction with disordered linker proteins that show little conservation between species. We developed a sequence-independent bioinformatic pipeline to identify linker proteins in green algae. We report the linker from Chlorella and demonstrate that it binds a conserved site on the Rubisco large subunit. We show that the Chlorella linker phase separates Chlamydomonas Rubisco and that despite their separation by ~800 million years of evolution, the Chlorella linker can support the formation of a functional pyrenoid in Chlamydomonas. This cross-species reactivity extends to plants, with the Chlorella linker able to drive condensation of some native plant Rubiscos in vitro and in planta. Our results represent an exciting frontier for pyrenoid engineering in plants, which is modelled to increase crop yields.

PubMed: 39384944
DOI: 10.1038/s41477-024-01812-x

実験手法

ELECTRON MICROSCOPY (2.77 Å)