8PYX

Amide bond synthetase from Streptomyces hindustanus K492H mutant in complex with Adenosine

8PYX の概要

• エントリーDOI: 10.2210/pdb8pyx/pdb
• 分子名称: Fatty-acyl-CoA synthase, SULFATE ION, ADENOSINE, ... (4 entities in total)
• 機能のキーワード: amide, ligase, amide bond synthetase, atp
• 由来する生物種: Streptoalloteichus hindustanus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110011.68

構造登録者

Tang, Q.,Grogan, G. (登録日: 2023-07-26, 公開日: 2024-02-07, 最終更新日: 2024-05-01)

主引用文献

Tang, Q.,Petchey, M.,Rowlinson, B.,Burden, T.J.,Fairlamb, I.J.S.,Grogan, G.
Broad Spectrum Enantioselective Amide Bond Synthetase from Streptoalloteichus hindustanus.
Acs Catalysis, 14:1021-1029, 2024

PubMed Abstract: The synthesis of amide bonds is one of the most frequently performed reactions in pharmaceutical synthesis, but the requirement for stoichiometric quantities of coupling agents and activated substrates in established methods has prompted interest in biocatalytic alternatives. Amide Bond Synthetases (ABSs) actively catalyze both the ATP-dependent adenylation of carboxylic acid substrates and their subsequent amidation using an amine nucleophile, both within the active site of the enzyme, enabling the use of only a small excess of the amine partner. We have assessed the ability of an ABS from (ShABS) to couple a range of carboxylic acid substrates and amines to form amine products. ShABS displayed superior activity to a previously studied ABS, McbA, and a remarkable complementary substrate specificity that included the enantioselective formation of a library of amides from racemic acid and amine coupling partners. The X-ray crystallographic structure of ShABS has permitted mutational mapping of the carboxylic acid and amine binding sites, revealing key roles for L207 and F246 in determining the enantioselectivity of the enzyme with respect to chiral acid and amine substrates. ShABS was applied to the synthesis of pharmaceutical amides, including ilepcimide, lazabemide, trimethobenzamide, and cinepazide, the last with 99% conversion and 95% isolated yield. These findings provide a blueprint for enabling a contemporary pharmaceutical synthesis of one of the most significant classes of small molecule drugs using biocatalysis.

PubMed: 38269041
DOI: 10.1021/acscatal.3c05656

実験手法

X-RAY DIFFRACTION (2.02 Å)