8PX7
Structure of Bacterial Multidrug Efflux transporter AcrB, solved at wavelength 3.02 A
Summary for 8PX7
| Entry DOI | 10.2210/pdb8px7/pdb |
| Descriptor | Multidrug efflux pump subunit AcrB (1 entity in total) |
| Functional Keywords | membrane protein, efflux pump |
| Biological source | Escherichia coli K-12 |
| Total number of polymer chains | 1 |
| Total formula weight | 114217.74 |
| Authors | El Omari, K.,Duman, R.,Mykhaylyk, V.,Orr, C.,Qu, F.,Beis, K.,Wagner, A. (deposition date: 2023-07-22, release date: 2023-10-25) |
| Primary citation | El Omari, K.,Duman, R.,Mykhaylyk, V.,Orr, C.M.,Latimer-Smith, M.,Winter, G.,Grama, V.,Qu, F.,Bountra, K.,Kwong, H.S.,Romano, M.,Reis, R.I.,Vogeley, L.,Vecchia, L.,Owen, C.D.,Wittmann, S.,Renner, M.,Senda, M.,Matsugaki, N.,Kawano, Y.,Bowden, T.A.,Moraes, I.,Grimes, J.M.,Mancini, E.J.,Walsh, M.A.,Guzzo, C.R.,Owens, R.J.,Jones, E.Y.,Brown, D.G.,Stuart, D.I.,Beis, K.,Wagner, A. Experimental phasing opportunities for macromolecular crystallography at very long wavelengths. Commun Chem, 6:219-219, 2023 Cited by PubMed Abstract: Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing. PubMed: 37828292DOI: 10.1038/s42004-023-01014-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.4 Å) |
Structure validation
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