8PVF

Structure of GAPDH determined by cryoEM at 100 keV

Summary for 8PVF

• Entry DOI: 10.2210/pdb8pvf/pdb
• EMDB information: 17964
• Descriptor: Glyceraldehyde-3-phosphate dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (2 entities in total)
• Functional Keywords: gapdh, oxidoreductase
• Biological source: Cryptosporidium parvum
• Total number of polymer chains: 1
• Total formula weight: 38722.91

Authors

McMullan, G.,Naydenova, K.,Mihaylov, D.,Peet, M.J.,Wilson, H.,Yamashita, K.,Dickerson, J.L.,Chen, S.,Cannone, G.,Lee, Y.,Hutchings, K.A.,Gittins, O.,Sobhy, M.,Wells, T.,El-Gomati, M.M.,Dalby, J.,Meffert, M.,Schulze-Briese, C.,Henderson, R.,Russo, C.J. (deposition date: 2023-07-17, release date: 2023-11-29, Last modification date: 2023-12-06)

Primary citation

McMullan, G.,Naydenova, K.,Mihaylov, D.,Yamashita, K.,Peet, M.J.,Wilson, H.,Dickerson, J.L.,Chen, S.,Cannone, G.,Lee, Y.,Hutchings, K.A.,Gittins, O.,Sobhy, M.A.,Wells, T.,El-Gomati, M.M.,Dalby, J.,Meffert, M.,Schulze-Briese, C.,Henderson, R.,Russo, C.J.
Structure determination by cryoEM at 100 keV.
Proc.Natl.Acad.Sci.USA, 120:e2312905120-e2312905120, 2023

PubMed Abstract: Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specimens and biological molecular structure determination in hours. We demonstrate its capabilities by determining the structures of eleven different specimens, ranging in size from 140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a microscope designed specifically for high-efficiency, on-the-spot imaging of biological molecules will expand structural biology to a wide range of previously intractable problems.

PubMed: 38011573
DOI: 10.1073/pnas.2312905120

Experimental method

ELECTRON MICROSCOPY (2.9 Å)