8PVD

Structure of catalase determined by cryoEM at 100 keV

8PVD の概要

• エントリーDOI: 10.2210/pdb8pvd/pdb
• EMDBエントリー: 17962
• 分子名称: Catalase, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61198.90

構造登録者

McMullan, G.,Naydenova, K.,Mihaylov, D.,Peet, M.J.,Wilson, H.,Yamashita, K.,Dickerson, J.L.,Chen, S.,Cannone, G.,Lee, Y.,Hutchings, K.A.,Gittins, O.,Sobhy, M.,Wells, T.,El-Gomati, M.M.,Dalby, J.,Meffert, M.,Schulze-Briese, C.,Henderson, R.,Russo, C.J. (登録日: 2023-07-17, 公開日: 2023-11-29, 最終更新日: 2023-12-06)

主引用文献

McMullan, G.,Naydenova, K.,Mihaylov, D.,Yamashita, K.,Peet, M.J.,Wilson, H.,Dickerson, J.L.,Chen, S.,Cannone, G.,Lee, Y.,Hutchings, K.A.,Gittins, O.,Sobhy, M.A.,Wells, T.,El-Gomati, M.M.,Dalby, J.,Meffert, M.,Schulze-Briese, C.,Henderson, R.,Russo, C.J.
Structure determination by cryoEM at 100 keV.
Proc.Natl.Acad.Sci.USA, 120:e2312905120-e2312905120, 2023

PubMed Abstract: Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specimens and biological molecular structure determination in hours. We demonstrate its capabilities by determining the structures of eleven different specimens, ranging in size from 140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a microscope designed specifically for high-efficiency, on-the-spot imaging of biological molecules will expand structural biology to a wide range of previously intractable problems.

PubMed: 38011573
DOI: 10.1073/pnas.2312905120

実験手法

ELECTRON MICROSCOPY (3.4 Å)