8PUR

DexyHemoglobin structure from serial synchrotron crystallography with fixed target

Summary for 8PUR

• Entry DOI: 10.2210/pdb8pur/pdb
• Descriptor: Hemoglobin, alpha 1, Hemoglobin subunit beta, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• Functional Keywords: horse hemoglobin, ssx, fixed target, oxygen transport
• Biological source: Equus caballus (horse); More
• Total number of polymer chains: 2
• Total formula weight: 32387.53

Authors

Bjelcic, M.,Sigfridsson Clauss, K.,Aurelius, O.,Milas, M.,Nan, J.,Ursby, T. (deposition date: 2023-07-17, release date: 2023-10-18, Last modification date: 2023-11-08)

Primary citation

Bjelcic, M.,Sigfridsson Clauss, K.G.V.,Aurelius, O.,Milas, M.,Nan, J.,Ursby, T.
Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes.
Acta Crystallogr D Struct Biol, 79:1018-1025, 2023

PubMed Abstract: In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a `sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV-Vis absorption spectroscopy.

PubMed: 37860963
DOI: 10.1107/S205979832300880X

Experimental method

X-RAY DIFFRACTION (1.85 Å)