8PUQ

MetHemoglobin structure from serial synchrotron crystallography with fixed target

8PUQ の概要

• エントリーDOI: 10.2210/pdb8puq/pdb
• 分子名称: Hemoglobin subunit alpha, Hemoglobin subunit beta, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: horse hemoglobin, ssx, fixed target, oxygen transport
• 由来する生物種: Equus caballus (horse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32246.33

構造登録者

Bjelcic, M.,Sigfridsson Clauss, K.,Aurelius, O.,Milas, M.,Nan, J.,Ursby, T. (登録日: 2023-07-17, 公開日: 2023-10-18, 最終更新日: 2023-11-08)

主引用文献

Bjelcic, M.,Sigfridsson Clauss, K.G.V.,Aurelius, O.,Milas, M.,Nan, J.,Ursby, T.
Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes.
Acta Crystallogr D Struct Biol, 79:1018-1025, 2023

PubMed Abstract: In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a `sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV-Vis absorption spectroscopy.

PubMed: 37860963
DOI: 10.1107/S205979832300880X

実験手法

X-RAY DIFFRACTION (1.95 Å)