8PTP

Structure of Rho pentamer in complex with Rof

8PTP の概要

• エントリーDOI: 10.2210/pdb8ptp/pdb
• 関連するPDBエントリー: 8PTM
• EMDBエントリー: 17877
• 分子名称: Transcription termination factor Rho, Protein rof (2 entities in total)
• 機能のキーワード: transcription termination factor rho inhibition of termination sm-like protein rof rho regulation, transcription
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 273825.87

構造登録者

Said, N.,Hilal, T.,Wahl, M.C. (登録日: 2023-07-14, 公開日: 2024-04-17, 最終更新日: 2024-04-24)

主引用文献

Said, N.,Finazzo, M.,Hilal, T.,Wang, B.,Selinger, T.L.,Gjorgjevikj, D.,Artsimovitch, I.,Wahl, M.C.
Sm-like protein Rof inhibits transcription termination factor rho by binding site obstruction and conformational insulation.
Nat Commun, 15:3186-3186, 2024

PubMed Abstract: Transcription termination factor ρ is a hexameric, RNA-dependent NTPase that can adopt active closed-ring and inactive open-ring conformations. The Sm-like protein Rof, a homolog of the RNA chaperone Hfq, inhibits ρ-dependent termination in vivo but recapitulation of this activity in vitro has proven difficult and the precise mode of Rof action is presently unknown. Here, our cryo-EM structures of ρ-Rof and ρ-RNA complexes show that Rof undergoes pronounced conformational changes to bind ρ at the protomer interfaces, undercutting ρ conformational dynamics associated with ring closure and occluding extended primary RNA-binding sites that are also part of interfaces between ρ and RNA polymerase. Consistently, Rof impedes ρ ring closure, ρ-RNA interactions and ρ association with transcription elongation complexes. Structure-guided mutagenesis coupled with functional assays confirms that the observed ρ-Rof interface is required for Rof-mediated inhibition of cell growth and ρ-termination in vitro. Bioinformatic analyses reveal that Rof is restricted to Pseudomonadota and that the ρ-Rof interface is conserved. Genomic contexts of rof differ between Enterobacteriaceae and Vibrionaceae, suggesting distinct modes of Rof regulation. We hypothesize that Rof and other cellular anti-terminators silence ρ under diverse, but yet to be identified, stress conditions when unrestrained transcription termination by ρ may be detrimental.

PubMed: 38622114
DOI: 10.1038/s41467-024-47439-6

実験手法

ELECTRON MICROSCOPY (3 Å)