8PSH

HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE

Summary for 8PSH

• Entry DOI: 10.2210/pdb8psh/pdb
• Related: 8DRH
• Descriptor: DNA (5'-D(*DGP*(SC)P*(GS)P*(PST)P*(SC)P*(AS)P*(GS)P*(GS))-3'), RNA (5'-R(*CP*CP*UP*GP*AP*CP*GP*C)-3') (2 entities in total)
• Functional Keywords: oligonucleotide, dna-rna hybrid, phosphorothioate, thionucleotide, antisense
• Total number of polymer chains: 2
• Total formula weight: 5081.64

Authors

Bachelin, M.,Hessler, G.,Kurz, G.,Hacia, J.G.,Dervan, P.B.,Kessler, H. (deposition date: 1997-10-13, release date: 1998-05-27, Last modification date: 2024-05-22)

Primary citation

Bachelin, M.,Hessler, G.,Kurz, G.,Hacia, J.G.,Dervan, P.B.,Kessler, H.
Structure of a Stereoregular Phosphorothioate DNA/RNA Duplex
Nat.Struct.Biol., 5:271-276, 1998

PubMed Abstract: In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.

PubMed: 9546216
DOI: 10.1038/nsb0498-271

Experimental method

SOLUTION NMR