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8PS4

Escherichia coli SduA complex

8PS4 の概要
エントリーDOI10.2210/pdb8ps4/pdb
EMDBエントリー17844
分子名称Shedu effector protein, MAGNESIUM ION (2 entities in total)
機能のキーワードsdua, shedu, prokaryotic immune system, immune system
由来する生物種Escherichia coli KTE10
タンパク質・核酸の鎖数4
化学式量合計189909.70
構造登録者
Loeff, L.,Jinek, M. (登録日: 2023-07-13, 公開日: 2025-01-22, 最終更新日: 2025-02-19)
主引用文献Loeff, L.,Walter, A.,Rosalen, G.T.,Jinek, M.
DNA end sensing and cleavage by the Shedu anti-phage defense system.
Cell, 188:721-, 2025
Cited by
PubMed Abstract: The detection of molecular patterns associated with invading pathogens is a hallmark of innate immune systems. Prokaryotes deploy sophisticated host defense mechanisms in innate anti-phage immunity. Shedu is a single-component defense system comprising a putative nuclease SduA. Here, we report cryoelectron microscopy (cryo-EM) structures of apo- and double-stranded DNA (dsDNA)-bound tetrameric SduA assemblies, revealing that the N-terminal domains of SduA form a clamp that recognizes free DNA ends. End binding positions the DNA over the PD-(D/E)XK nuclease domain, resulting in dsDNA nicking at a fixed distance from the 5' end. The end-directed DNA nicking activity of Shedu prevents propagation of linear DNA in vivo. Finally, we show that phages escape Shedu immunity by suppressing their recombination-dependent DNA replication pathway. Taken together, these results define the antiviral mechanism of Shedu systems, underlining the paradigm that recognition of pathogen-specific nucleic acid structures is a conserved feature of innate immunity across all domains of life.
PubMed: 39742808
DOI: 10.1016/j.cell.2024.11.030
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (2.95 Å)
構造検証レポート
Validation report summary of 8ps4
検証レポート(詳細版)ダウンロードをダウンロード

239149

件を2025-07-23に公開中

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