8PS4
Escherichia coli SduA complex
8PS4 の概要
エントリーDOI | 10.2210/pdb8ps4/pdb |
EMDBエントリー | 17844 |
分子名称 | Shedu effector protein, MAGNESIUM ION (2 entities in total) |
機能のキーワード | sdua, shedu, prokaryotic immune system, immune system |
由来する生物種 | Escherichia coli KTE10 |
タンパク質・核酸の鎖数 | 4 |
化学式量合計 | 189909.70 |
構造登録者 | |
主引用文献 | Loeff, L.,Walter, A.,Rosalen, G.T.,Jinek, M. DNA end sensing and cleavage by the Shedu anti-phage defense system. Cell, 188:721-, 2025 Cited by PubMed Abstract: The detection of molecular patterns associated with invading pathogens is a hallmark of innate immune systems. Prokaryotes deploy sophisticated host defense mechanisms in innate anti-phage immunity. Shedu is a single-component defense system comprising a putative nuclease SduA. Here, we report cryoelectron microscopy (cryo-EM) structures of apo- and double-stranded DNA (dsDNA)-bound tetrameric SduA assemblies, revealing that the N-terminal domains of SduA form a clamp that recognizes free DNA ends. End binding positions the DNA over the PD-(D/E)XK nuclease domain, resulting in dsDNA nicking at a fixed distance from the 5' end. The end-directed DNA nicking activity of Shedu prevents propagation of linear DNA in vivo. Finally, we show that phages escape Shedu immunity by suppressing their recombination-dependent DNA replication pathway. Taken together, these results define the antiviral mechanism of Shedu systems, underlining the paradigm that recognition of pathogen-specific nucleic acid structures is a conserved feature of innate immunity across all domains of life. PubMed: 39742808DOI: 10.1016/j.cell.2024.11.030 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (2.95 Å) |
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