8POW

Crystal Structure of the C19G variant of the membrane-bound [NiFe]-Hydrogenase from Cupriavidus necator in the air-oxidized state at 1.61 A Resolution.

8POW の概要

• エントリーDOI: 10.2210/pdb8pow/pdb
• 関連するPDBエントリー: 3RGW 4IUB 4IUC 4IUD 4TTT 5D51 5MDJ 5MDK 5MDL 7ODG 7ODH
• 分子名称: Uptake hydrogenase large subunit, Uptake hydrogenase small subunit, NI-FE OXIDIZED ACTIVE CENTER, ... (9 entities in total)
• 機能のキーワード: [nife]-hydrogenase, hydrogenase, oxygen-tolerance, hydrogen catalysis, knallgasbacteria, proteobacteria, metalloenzyme, bimetallic, ni-fe active site, [4fe-3s], proximal cluster, aerobic hydrogen bacteria, oxidoreductase, electron transfer, metalloprotein, catalytic center, membrane, membrane-bound, oxidoreductase-oxidoreductase complex, electron relay, cubane cluster, high potential, cluster tuning, electron transport, as-isolated state, air-oxidized
• 由来する生物種: Cupriavidus necator H16; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104584.45

構造登録者

Kalms, J.,Schmidt, A.,Scheerer, P. (登録日: 2023-07-05, 公開日: 2023-11-15)

• 主引用文献: Schmidt, A.,Kalms, J.,Lorent, C.,Katz, S.,Frielingsdorf, S.,Evans, R.M.,Fritsch, J.,Siebert, E.,Teutloff, C.,Armstrong, F.A.,Zebger, I.,Lenz, O.,Scheerer, P.; Stepwise conversion of the Cys 6 [4Fe-3S] to a Cys 4 [4Fe-4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase.; Chem Sci, 14:11105-11120, 2023; PubMed: 37860641; DOI: 10.1039/d3sc03739h

実験手法

X-RAY DIFFRACTION (1.61 Å)